論文 - 詳細
| RRC ID | 39316 |
|---|---|
| 著者 | Ishihara K, Satoh I, Nittoh T, Kanaya T, Okazaki H, Suzuki T, Koyama T, Sakamoto T, Ide T, Ohuchi K. |
| タイトル | Preparation of recombinant rat interleukin-5 by baculovirus expression system and analysis of its biological activities. |
| ジャーナル | Biochim Biophys Acta |
| Abstract |
Rat interleukin-5 (IL-5) cDNA was subcloned from peritoneal cells collected 4 h after intraperitoneal injection of Ascaris suum antigen solution into the immunized rats. Cysteine proteinase-deleted (CPd) rat IL-5 recombinant virus was constructed by inserting rat IL-5 cDNA into CPd virus having a deletion in the cysteine proteinase gene of the silkworm Bombyx mori nuclear polyhedrosis virus. On infection with the CPd rat IL-5 recombinant virus, the silkworm B. mori larvae produced rat IL-5 as a dimeric form in hemolymph. Recombinant rat IL-5 was purified more than 95.5% by anion-exchange chromatography and hydrophobic chromatography. The purified recombinant rat IL-5 promoted the proliferation of T88-M cells in a concentration-dependent manner, and its effect was inhibited by an anti-murine IL-5 neutralizing polyclonal antibody. When bone marrow cells from normal rats were incubated with recombinant rat IL-5 in medium containing methylcellulose, the colony formation by eosinophilic cells was induced. Furthermore, when rat peritoneal eosinophils were incubated with recombinant rat IL-5, the spontaneous decrease in the eosinophil viability was inhibited in time- and concentration-dependent manners. In addition, the recombinant rat IL-5-induced eosinophil survival was inhibited by an anti-murine IL-5 neutralizing polyclonal antibody. These findings suggest that rat IL-5 acts as B-cell growth factor II (BCGF-II), eosinophil differentiation factor (EDF), and eosinophil survival-enhancing factor. |
| 巻・号 | 1451(1) |
| ページ | 48-58 |
| 公開日 | 1999-8-12 |
| DOI | 10.1016/s0167-4889(99)00090-7 |
| PII | S0167-4889(99)00090-7 |
| PMID | 10446387 |
| MeSH | Animals B-Lymphocytes / drug effects Baculoviridae / genetics Blotting, Western Bombyx / metabolism Cell Differentiation / drug effects Cell Division / drug effects Cell Line Cell Survival / drug effects Cloning, Molecular Colony-Forming Units Assay Electrophoresis, Polyacrylamide Gel Eosinophils / drug effects Hemolymph / metabolism Interleukin-5 / genetics Interleukin-5 / isolation & purification Interleukin-5 / metabolism* Rats Recombinant Proteins / metabolism |
| IF | 3.411 |
| 引用数 | 13 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | T88-M(RCB0792) |