RRC ID 39320
Author Okamoto K, Tanaka H, Ogawa H, Makino Y, Eguchi H, Hayashi S, Yoshikawa N, Poellinger L, Umesono K, Makino I.
Title Redox-dependent regulation of nuclear import of the glucocorticoid receptor.
Journal J Biol Chem
Abstract A number of transcription factors including the glucocorticoid receptor (GR) are regulated in a redox-dependent fashion. We have previously reported that the functional activity of the GR is suppressed under oxidative conditions and restored in the presence of reducing reagents. In the present study, we have used a chimeric human GR fused to the Aequorea green fluorescent protein and demonstrated that both ligand-dependent and -independent nuclear translocation of the GR is impaired under oxidative conditions in living cells. Substitution of Cys-481 for Ser within NL1 of the human GR resulted in reduction of sensitivity to oxidative treatment, strongly indicating that Cys-481 is one of the target amino acids for redox regulation of the receptor. Taken together, we may conclude that redox-dependent regulation of nuclear translocation of the GR constitutes an important mechanism for modulation of glucocorticoid-dependent signal transduction.
Volume 274(15)
Pages 10363-71
Published 1999-4-9
DOI 10.1074/jbc.274.15.10363
PMID 10187825
MeSH Amino Acid Sequence Amino Acid Substitution Animals Biological Transport / drug effects CHO Cells COS Cells Cell Nucleus / metabolism Cricetinae Cysteine / metabolism Green Fluorescent Proteins Humans Hydrogen Peroxide / pharmacology Ligands Luminescent Proteins / genetics Luminescent Proteins / metabolism Molecular Sequence Data Oxidation-Reduction Oxidative Stress Receptors, Glucocorticoid / genetics Receptors, Glucocorticoid / metabolism* Recombinant Fusion Proteins / metabolism Serine / metabolism Signal Transduction Zinc Fingers
IF 4.106
Times Cited 132
Human and Animal Cells