| RRC ID |
39335
|
| 著者 |
Nukaya I, Yasumoto M, Iwasaki T, Ideno M, Sette A, Celis E, Takesako K, Kato I.
|
| タイトル |
Identification of HLA-A24 epitope peptides of carcinoembryonic antigen which induce tumor-reactive cytotoxic T lymphocyte.
|
| ジャーナル |
Int J Cancer
|
| Abstract |
Carcinoembryonic antigen (CEA), which is expressed in several cancer types, is a potential target for specific immunotherapy. HLA-A24 is the most frequent allele among Japanese and is also frequently present in Asians and Caucasians. We tested CEA-encoded HLA-A24 binding peptides for their capacity to elicit anti-tumor cytotoxic T lymphocytes (CTL) in vitro. For this purpose, we used CD8+ T lymphocytes from peripheral blood mononuclear cells (PBMC) of a healthy donor and autologous peptide-pulsed dendritic cells as antigen-presenting cells. This approach enabled us to identify 2 peptides, QYSWFVNGTF and TYACFVSNL, which were capable of eliciting CTL lines that lysed tumor cells expressing HLA-A24 and CEA. The cytotoxicity to tumor cells by the CTL lines was antigen-specific since it was inhibited by peptide-pulsed cold target cells as well as by anti-class I major histocompatibility complex (MHC) and anti-CD3 monoclonal antibodies (MAbs). The antigen specificity of the 2 CTL lines was examined using several tumor cell lines of various origins and for their peptide-dose responses. The identification of these novel CEA epitopes for CTL offers the opportunity to design and develop epitope-based immunotherapeutic approaches for treating HLA-A24+ patients with tumors that express CEA.
|
| 巻・号 |
80(1)
|
| ページ |
92-7
|
| 公開日 |
1999-1-5
|
| DOI |
10.1002/(sici)1097-0215(19990105)80:1<92::aid-ijc18>3.0.co;2-m
|
| PII |
10.1002/(SICI)1097-0215(19990105)80:1<92::AID-IJC18>3.0.CO;2-M
|
| PMID |
9935237
|
| MeSH |
Amino Acid Sequence
Antibodies, Monoclonal / pharmacology
Asian People / genetics
Carcinoembryonic Antigen / chemistry
Carcinoembryonic Antigen / genetics*
Cytotoxicity, Immunologic*
Dendritic Cells / immunology
Digestive System Neoplasms / immunology
Epitopes / chemistry
Epitopes / genetics
Epitopes / immunology
HLA-A Antigens / genetics*
HLA-A Antigens / immunology*
HLA-A24 Antigen
Histocompatibility Antigens Class I / immunology
Humans
Interferon-gamma / pharmacology
Japan
Lymphoma, B-Cell / immunology
Melanoma / immunology
T-Lymphocytes, Cytotoxic / immunology*
Tumor Cells, Cultured
White People / genetics
|
| IF |
5.145
|
| 引用数 |
90
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
GT3TKB(RCB0885) |
