論文 - 詳細
| RRC ID | 39370 |
|---|---|
| 著者 | Hamasuna R, Kataoka H, Moriyama T, Itoh H, Seiki M, Koono M. |
| タイトル | Regulation of matrix metalloproteinase-2 (MMP-2) by hepatocyte growth factor/scatter factor (HGF/SF) in human glioma cells: HGF/SF enhances MMP-2 expression and activation accompanying up-regulation of membrane type-1 MMP. |
| ジャーナル | Int J Cancer |
| Abstract |
Hepatocyte growth factor/scatter factor (HGF/SF) contributes to the malignant progression of human gliomas. We investigated the effect of HGF/SF on matrix metalloproteinase-2 (MMP-2), membrane type 1 matrix metalloproteinase (MT1-MMP) and tissue inhibitors of metalloproteinases (TIMPs), expressions of c-Met/HGF receptor-positive human glioblastoma cells. Treatment of U251 human glioblastoma cells with HGF/SF resulted in enhanced secretion of MMP-2 with an increased level of the active form. This was accompanied by enhanced expression (2.5-fold) of mRNA specific for MMP-2. The stimulatory effect of HGF/SF on MMP-2 expression did not occur in the presence of herbimycin A, a protein tyrosine kinase inhibitor. MT1 -MMP, a cell-surface activator of proMMP-2, was also up-regulated by HGF/SF in a dose-dependent manner. By contrast, the level of TIMP- 1 mRNAs was not altered significantly and that of TIMP-2 was reduced mildly by the HGF/SF treatment, suggesting that HGF/SF may eventually modulate a balance between MMP-2 and TIMPs in favor of the proteinase activity in the glioma cell microenvironment. HGF/SF also stimulated MMP-2 expression of other glioblastoma cell lines. Since glioblastomas frequently co-express HGF/SF and its receptor, our results suggest that HGF/SF might contribute to the invasiveness of glioblastoma cells through autocrine induction of MMP-2 expression and activation. |
| 巻・号 | 82(2) |
| ページ | 274-81 |
| 公開日 | 1999-7-19 |
| DOI | 10.1002/(sici)1097-0215(19990719)82:2<274::aid-ijc19>3.0.co;2-2 |
| PII | 10.1002/(SICI)1097-0215(19990719)82:2<274::AID-IJC19>3.0.CO;2-2 |
| PMID | 10389763 |
| MeSH | Benzoquinones Brain Neoplasms / enzymology Brain Neoplasms / genetics Brain Neoplasms / pathology* Disease Progression Enzyme Induction / drug effects Enzyme Inhibitors / pharmacology Epidermal Growth Factor / pharmacology Gelatinases / biosynthesis* Gelatinases / genetics Gene Expression Regulation, Neoplastic / drug effects* Glioblastoma / enzymology Glioblastoma / genetics Glioblastoma / pathology Glioma / enzymology Glioma / genetics Glioma / pathology* Hepatocyte Growth Factor / pharmacology* Humans Lactams, Macrocyclic Matrix Metalloproteinase 2 Matrix Metalloproteinases, Membrane-Associated Metalloendopeptidases / biosynthesis* Metalloendopeptidases / genetics Neoplasm Invasiveness Neoplasm Proteins / biosynthesis* Neoplasm Proteins / genetics Protein-Tyrosine Kinases / antagonists & inhibitors Quinones / pharmacology RNA, Messenger / biosynthesis RNA, Messenger / genetics RNA, Neoplasm / biosynthesis RNA, Neoplasm / genetics Recombinant Proteins / pharmacology Rifabutin / analogs & derivatives Signal Transduction / drug effects Stimulation, Chemical Tumor Cells, Cultured |
| IF | 5.145 |
| 引用数 | 58 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | U251(RCB0461) |