RRC ID 39378
著者 Fujii M, Takeda K, Imamura T, Aoki H, Sampath TK, Enomoto S, Kawabata M, Kato M, Ichijo H, Miyazono K.
タイトル Roles of bone morphogenetic protein type I receptors and Smad proteins in osteoblast and chondroblast differentiation.
ジャーナル Mol Biol Cell
Abstract The biological effects of type I serine/threonine kinase receptors and Smad proteins were examined using an adenovirus-based vector system. Constitutively active forms of bone morphogenetic protein (BMP) type I receptors (BMPR-IA and BMPR-IB; BMPR-I group) and those of activin receptor-like kinase (ALK)-1 and ALK-2 (ALK-1 group) induced alkaline phosphatase activity in C2C12 cells. Receptor-regulated Smads (R-Smads) that act in the BMP pathways, such as Smad1 and Smad5, also induced the alkaline phosphatase activity in C2C12 cells. BMP-6 dramatically enhanced alkaline phosphatase activity induced by Smad1 or Smad5, probably because of the nuclear translocation of R-Smads triggered by the ligand. Inhibitory Smads, i.e., Smad6 and Smad7, repressed the alkaline phosphatase activity induced by BMP-6 or the type I receptors. Chondrogenic differentiation of ATDC5 cells was induced by the receptors of the BMPR-I group but not by those of the ALK-1 group. However, kinase-inactive forms of the receptors of the ALK-1 and BMPR-I groups blocked chondrogenic differentiation. Although R-Smads failed to induce cartilage nodule formation, inhibitory Smads blocked it. Osteoblast differentiation induced by BMPs is thus mediated mainly via the Smad-signaling pathway, whereas chondrogenic differentiation may be transmitted by Smad-dependent and independent pathways.
巻・号 10(11)
ページ 3801-13
公開日 1999-11-1
DOI 10.1091/mbc.10.11.3801
PMID 10564272
PMC PMC25680
MeSH Activin Receptors Adenoviridae / genetics Alkaline Phosphatase / metabolism Animals Bone Morphogenetic Protein Receptors, Type I Cell Differentiation Cell Line Chondrocytes DNA-Binding Proteins / metabolism* Genetic Vectors Histocytochemistry Mice Osteoblasts Phosphoproteins / metabolism Phosphorylation Protein Serine-Threonine Kinases / metabolism* Receptors, Growth Factor / metabolism* Smad Proteins Smad1 Protein Smad5 Protein Smad6 Protein Smad7 Protein Trans-Activators / metabolism*
IF 3.791
引用数 339
WOS 分野 CELL BIOLOGY
リソース情報
ヒト・動物細胞 ATDC5(RCB0565)