| RRC ID |
39390
|
| 著者 |
Takekoshi K, Isobe K, Nomura F, Motooka M, Nanmoku T, Nakai T.
|
| タイトル |
Effects of PAMP on mRNAs coding for catecholamine-synthesizing enzymes in PC12 cells.
|
| ジャーナル |
Life Sci
|
| Abstract |
Proadrenomedullin N-terminal 20 peptide (PAMP) is a novel hypotensive peptide found in the N-terminal portion of the precursor of adrenomedullin (AM). Although PAMP and AM originate from the same precursor and exert both a potent hypotensive action, they seem to control blood pressure through different mechanisms. To gain new insight into the anticholinergic actions of PAMP, we determined the effects of PAMP on the tyrosine hydroxylase (TH)- and dopamine beta-hydroxylase (DBH) mRNA expression in the rat pheochromocytoma cell line PC12 stimulated by nicotine. PAMP (> or =1 microM) significantly inhibited the nicotine-induced increases of TH- and DBH mRNA expression in a concentration-dependent manner. Also, PAMP at the concentrations (> or =1 microM) significantly inhibited nicotine-induced cyclic adenosine monophosphate (cAMP) production. These results indicate that the anticholinergic hypotensive actions of PAMP can be explained, at least in part, by its inhibition of the expression of mRNAs coding for catecholamine-synthesizing enzymes, and that the inhibitory effect is mediated by the cAMP/protein kinase A pathway.
|
| 巻・号 |
65(8)
|
| ページ |
771-81
|
| 公開日 |
1999-1-1
|
| DOI |
10.1016/s0024-3205(99)00304-5
|
| PII |
S0024320599003045
|
| PMID |
10466743
|
| MeSH |
Adrenomedullin
Animals
Cyclic AMP / metabolism
Dopamine beta-Hydroxylase / genetics*
Hypotension / drug therapy
Nicotine / pharmacology
PC12 Cells / drug effects
PC12 Cells / enzymology
Peptide Fragments / pharmacology*
Peptides*
Proteins / pharmacology*
RNA, Messenger / metabolism*
Rats
Tyrosine 3-Monooxygenase / genetics*
|
| IF |
3.647
|
| 引用数 |
9
|
|
WOS 分野
|
MEDICINE, RESEARCH & EXPERIMENTAL
PHARMACOLOGY & PHARMACY
|
| リソース情報 |
| ヒト・動物細胞 |
PC-12(RCB0009) |
