RRC ID 39405
著者 Uchida H, Kiyokawa N, Taguchi T, Horie H, Fujimoto J, Takeda T.
タイトル Shiga toxins induce apoptosis in pulmonary epithelium-derived cells.
ジャーナル J Infect Dis
Abstract The effect of Shiga toxins (Stxs) produced by Escherichia coli on human lung epithelial cells was investigated. Specific antibodies for Stxs positively stained lung tissue from a patient who died of hemolytic uremic syndrome associated with Stx-producing E. coli (STEC) infection, indicating the deposition of Stxs in the lung. Binding experiments with normal lung tissue revealed apparent Stx binding to both vascular endothelium and to portions of the pulmonary epithelium. CD77-positive lung carcinoma cell lines, which are derived from lung epithelium, showed binding to Stx and a high susceptibility to Stxs, as determined by MTT assay. Consistent with our previous reports on renal tubular epithelium, apoptosis is involved in the Stx-mediated cytotoxicity of these lines. These data indicate that lung epithelium is another target for Stxs, and Stx-mediated injury to lung epithelial cells is thought to play an important role in the pathogenesis of pulmonary involvement associated with STEC infection.
巻・号 180(6)
ページ 1902-11
公開日 1999-12-1
DOI 10.1086/315131
PII JID990345
PMID 10558947
MeSH Antigens, CD Apoptosis* Bacterial Toxins / metabolism Bacterial Toxins / toxicity* Endothelium, Vascular / cytology Epithelial Cells / physiology Escherichia coli / metabolism* Escherichia coli Infections / microbiology* Escherichia coli Infections / pathology Female Fluorescent Antibody Technique Hemolytic-Uremic Syndrome / microbiology Humans Immunohistochemistry Infant Lung / cytology* Lung / metabolism Shiga Toxins Tumor Cells, Cultured
IF 5.022
引用数 32
WOS 分野 INFECTIOUS DISEASES IMMUNOLOGY MICROBIOLOGY
オルトメトリクス指標
オルトメトリクス指標項目
最多言及媒体 Patent(IFI CLAIMS)
各媒体での言及数の合計 1
過去6か月間でのオルトメトリクス指標の変動値 0.0
リソース情報
ヒト・動物細胞 A549(RCB0098) PC-14(RCB0446) RERF-LC-AI(RCB0444)