論文 - 詳細
| RRC ID | 39413 |
|---|---|
| 著者 | Uezono Y, Shibuya I, Ueda Y, Tanaka K, Oishi Y, Yanagihara N, Ueno S, Toyohira Y, Nakamura T, Yamashita H, Izumi F. |
| タイトル | Adrenomedullin increases intracellular Ca2+ and inositol 1,4,5-trisphosphate in human oligodendroglial cell line KG-1C. |
| ジャーナル | Brain Res |
| Abstract |
The effects of adrenomedullin (AM), a hypotensive peptide, were investigated in cultured human oligodendroglial cell line KG-1C. Human AM increased the intracellular Ca2+ concentration ([Ca2+]i) at concentrations greater than 10(-7) M. Human calcitonin gene-related peptide (CGRP), a peptide structurally related to AM, also increased [Ca2+]i with a potency similar to that of AM. AM increased [Ca2+]i in the absence of extracellular Ca2+. Further, AM increased inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) level in a concentration-dependent manner similar to that of AM-induced [Ca2+]i, suggesting that AM-induced elevation of [Ca2+]i is due to Ca2+ release from Ins(1,4,5)P3-sensitive stores. AM (10(-9) to 10(-6) M) increased cAMP in a concentration-dependent manner. Forskolin also increased cAMP, but did not mimic the [Ca2+]i-raising effect of AM. These findings suggest that functional AM receptors are present in oligodendroglial KG-1C cells and that AM increases [Ca2+]i through a mechanism independent of cAMP. |
| 巻・号 | 786(1-2) |
| ページ | 230-4 |
| 公開日 | 1998-3-9 |
| DOI | 10.1016/s0006-8993(97)01430-3 |
| PII | S0006-8993(97)01430-3 |
| PMID | 9555029 |
| MeSH | Adrenomedullin Calcitonin Gene-Related Peptide / pharmacology Calcium / metabolism* Colforsin / pharmacology Cyclic AMP / metabolism Humans Inositol 1,4,5-Trisphosphate / metabolism* Intracellular Fluid / metabolism* Oligodendroglia / drug effects* Oligodendroglia / metabolism* Peptides / pharmacology* Tumor Cells, Cultured |
| IF | 2.733 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | KG-1-C(RCB0270) |