| RRC ID |
39413
|
| 著者 |
Uezono Y, Shibuya I, Ueda Y, Tanaka K, Oishi Y, Yanagihara N, Ueno S, Toyohira Y, Nakamura T, Yamashita H, Izumi F.
|
| タイトル |
Adrenomedullin increases intracellular Ca2+ and inositol 1,4,5-trisphosphate in human oligodendroglial cell line KG-1C.
|
| ジャーナル |
Brain Res
|
| Abstract |
The effects of adrenomedullin (AM), a hypotensive peptide, were investigated in cultured human oligodendroglial cell line KG-1C. Human AM increased the intracellular Ca2+ concentration ([Ca2+]i) at concentrations greater than 10(-7) M. Human calcitonin gene-related peptide (CGRP), a peptide structurally related to AM, also increased [Ca2+]i with a potency similar to that of AM. AM increased [Ca2+]i in the absence of extracellular Ca2+. Further, AM increased inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) level in a concentration-dependent manner similar to that of AM-induced [Ca2+]i, suggesting that AM-induced elevation of [Ca2+]i is due to Ca2+ release from Ins(1,4,5)P3-sensitive stores. AM (10(-9) to 10(-6) M) increased cAMP in a concentration-dependent manner. Forskolin also increased cAMP, but did not mimic the [Ca2+]i-raising effect of AM. These findings suggest that functional AM receptors are present in oligodendroglial KG-1C cells and that AM increases [Ca2+]i through a mechanism independent of cAMP.
|
| 巻・号 |
786(1-2)
|
| ページ |
230-4
|
| 公開日 |
1998-3-9
|
| DOI |
10.1016/s0006-8993(97)01430-3
|
| PII |
S0006-8993(97)01430-3
|
| PMID |
9555029
|
| MeSH |
Adrenomedullin
Calcitonin Gene-Related Peptide / pharmacology
Calcium / metabolism*
Colforsin / pharmacology
Cyclic AMP / metabolism
Humans
Inositol 1,4,5-Trisphosphate / metabolism*
Intracellular Fluid / metabolism*
Oligodendroglia / drug effects*
Oligodendroglia / metabolism*
Peptides / pharmacology*
Tumor Cells, Cultured
|
| IF |
2.733
|
| リソース情報 |
| ヒト・動物細胞 |
KG-1-C(RCB0270) |
