| RRC ID |
39430
|
| 著者 |
Sakai K, Mohtai M, Shida J, Harimaya K, Benvenuti S, Brandi ML, Kukita T, Iwamoto Y.
|
| タイトル |
Fluid shear stress increases interleukin-11 expression in human osteoblast-like cells: its role in osteoclast induction.
|
| ジャーナル |
J Bone Miner Res
|
| Abstract |
It is unclear how mechanical stress influences bone cells. Mechanical stress causes fluid shear stress (FSS) in the bone. Osteoblast lineage cells are thought to sense FSS and regulate bone remodeling. We therefore investigated the effects of FSS on human osteoblast-like osteosarcoma cells: SaOS-2 cells in vitro. The conditioned medium of the SaOS-2 cells after 24 h of FSS (24 h-FSS CM) showed such osteoclastic phenotype inductions as significantly increasing the number of tartrate-resistant acid phosphatase (TRAP) positive multinuclear cells in rat bone marrow cells and TRAP-positive cells in human preosteoclastic cells: FLG 29.1 cells. An enzyme-linked immunosorbent assay showed interleukin-11 (IL-11) protein to increase 7-fold in the 24 h-FSS CM. A Northern analysis showed that IL-11 mRNA increased 4-fold in the SaOS-2 cells after 6 h-FSS; however, no IL-6 mRNA expression was detected. Furthermore, the anti-human IL-11 antibody significantly neutralized the osteoclastic phenotype induction of the 24 h-FSS CM. The IL-11 mRNA up-regulation in SaOS-2 cells by the 6 h-FSS was not inhibited by the anti-human transforming growth factor-beta1 antibody, but it was significantly inhibited by indomethacin. An enzymeimmunoassay showed prostaglandin E2 to increase 7-fold in the 1 h-FSS CM. These findings thus suggest that FSS induces osteoblasts to produce IL-11 (mediated by prostaglandins) and thus stimulates bone remodeling.
|
| 巻・号 |
14(12)
|
| ページ |
2089-98
|
| 公開日 |
1999-12-1
|
| DOI |
10.1359/jbmr.1999.14.12.2089
|
| PII |
jbm574
|
| PMID |
10620068
|
| MeSH |
Acid Phosphatase
Animals
Antibodies / pharmacology
Bone Remodeling / drug effects*
Culture Media, Conditioned
Dinoprostone / metabolism
Filaggrin Proteins
Gene Expression Regulation
Histocytochemistry
Humans
Indomethacin / pharmacology
Interleukin-11 / biosynthesis*
Interleukin-11 / genetics
Interleukin-11 / immunology
Isoenzymes
Osteoblasts / drug effects
Osteoblasts / metabolism*
RNA, Messenger / biosynthesis
Rats
Stress, Mechanical
Tartrate-Resistant Acid Phosphatase
Transforming Growth Factor beta / pharmacology
Tumor Cells, Cultured
Up-Regulation / drug effects
|
| IF |
5.854
|
| 引用数 |
30
|
|
WOS 分野
|
ENDOCRINOLOGY & METABOLISM
|
| リソース情報 |
| ヒト・動物細胞 |
Saos-2(RCB0428) |
