RRC ID 39431
Author Ichino N, Ishiguro H, Yamada K, Nishii K, Sawada H, Nagatsu T.
Title Nicotine withdrawal up-regulates c-Fos transcription in pheochromocytoma cells.
Journal Neurosci Res
Abstract The influence of nicotine on the expression of Fos family proteins, which specifically formed complexes with the AP-1 sequence, was assessed. mRNA for c-Fos, c-jun and jun-B were up-regulated at 0.5 h after nicotine treatment, elevated c-Fos also being apparent after withdrawal. Although nicotine failed to up-regulate the mRNA level of the fra-1 gene, the Fra- protein was highly expressed after both treatment and withdrawal. These results indicate that nicotine treatment may affect the transcriptional activity of many genes through c-Fos and c-Jun protein expression in neural cells, and that Fra-1 protein may make a contribution. These changes in immediately early genes (IEGs) may be associated with nicotine withdrawal symptoms.
Volume 35(1)
Pages 63-9
Published 1999-10
DOI 10.1016/s0168-0102(99)00069-3
PII S0168-0102(99)00069-3
PMID 10555165
MeSH Adrenal Gland Neoplasms Amino Acid Sequence Animals Antisense Elements (Genetics) Gene Expression Regulation, Neoplastic / drug effects Gene Expression Regulation, Neoplastic / physiology* Genes, fos* Genes, jun Humans Mice Molecular Sequence Data Nicotine / pharmacology* Oligonucleotide Probes PC12 Cells Pheochromocytoma Proto-Oncogene Proteins c-fos / chemistry Proto-Oncogene Proteins c-fos / genetics* Proto-Oncogene Proteins c-jun / genetics RNA, Messenger / genetics Rats Sequence Alignment Sequence Homology, Amino Acid Substance Withdrawal Syndrome Transcription, Genetic / drug effects
IF 2.071
Times Cited 10
Human and Animal Cells