| RRC ID |
39433
|
| 著者 |
Andoh K, Piao JH, Terashima K, Nakamura H, Sano K.
|
| タイトル |
Genomic structure and promoter analysis of the ecto-phosphodiesterase I gene (PDNP3) expressed in glial cells.
|
| ジャーナル |
Biochim Biophys Acta
|
| Abstract |
PDNP (phosphodiesterase I/nucleotide pyrophosphatase) is one of a series of ectoenzymes that are involved in hydrolysis of extracellular nucleotides. PDNP possesses ATPase (EC 3.6.1.3) and ATP pyrophosphatase (EC 3.6.1.8) activities. Mammalian PDNP consists of three closely related family proteins (PDNP1, -2, and -3), and they are expressed in different cell types and at different developmental stages. Rat PDNP3 is expressed in a subset of immature glial cells and in the alimentary tract. Human PDNP3 is expressed in glioma cells, prostate, and uterus, but not in the alimentary tract. We have cloned genomic DNA containing the whole coding region of the human PDNP3 gene and determined its exon-intron structure. The human PDNP3 gene spans over 60 kb and is organized into 25 exons and 24 introns. We determined the nucleotide sequence of the 5'-flanking region of human and rat PDNP3 genes. The upstream region of both species lacks a canonical TATA box and contains a putative binding site for CCAAT enhancer-binding proteins near the transcription start site. Promoter activity analysis of the 5'-flanking region revealed that the sequence around the CCAAT box is required for its transcriptional activity in 9L rat glioma cells. A gel shift assay demonstrated that 9L nuclear extract contains proteins that bind to this region.
|
| 巻・号 |
1446(3)
|
| ページ |
213-24
|
| 公開日 |
1999-9-3
|
| DOI |
10.1016/s0167-4781(99)00090-1
|
| PII |
S0167-4781(99)00090-1
|
| PMID |
10524196
|
| MeSH |
Animals
Base Sequence
Cell Line
Cloning, Molecular
Colon / metabolism
DNA, Complementary / chemistry
DNA, Complementary / isolation & purification
Exons
Humans
Intestine, Small / metabolism
Introns
Liver / metabolism
Molecular Sequence Data
Neuroglia / enzymology
Neuroglia / metabolism*
Pancreas / metabolism
Phosphodiesterase I
Phosphoric Diester Hydrolases / genetics*
Promoter Regions, Genetic
Pyrophosphatases / genetics*
Rats
Reverse Transcriptase Polymerase Chain Reaction
|
| IF |
3.411
|
| 引用数 |
20
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
COS-7(RCB0539) |
