| RRC ID |
39439
|
| Author |
Okubo MA, Chiba S, Nishikata T, Matsuno A, Hosoya H.
|
| Title |
Generation and characterization of a monoclonal antibody, mH1, raised against mitotic HeLa cells.
|
| Journal |
Dev Growth Differ
|
| Abstract |
Hybridoma cell lines were prepared from spleen cells of mouse immunized with mitotic HeLa cells. A monoclonal antibody (mH1), which intensively reacted with cleavage furrows of dividing HeLa cells in immunofluorescence, was obtained. In interphase, this antibody diffusely stained whole HeLa cells. Immunoelectron microscopy showed that mH1 antigens were localized at microvillus projections at the surface of dividing HeLa cells, but definite localization of that antigen was not observed in interphasic cells. Immunoblot analysis showed that mH1 is reactive to 42-kDa and 130-kDa components. Further, the 42-kDa component was identified as a gamma-actin homolog by N-terminal amino acid sequence analysis.
|
| Volume |
41(4)
|
| Pages |
381-9
|
| Published |
1999-8-1
|
| DOI |
10.1046/j.1440-169x.1999.00438.x
|
| PMID |
10466925
|
| MeSH |
Actins / chemistry
Actins / immunology
Amino Acid Sequence
Animals
Antibodies, Monoclonal / biosynthesis*
Antibodies, Monoclonal / chemistry
Antigens, Differentiation / immunology
Antigens, Differentiation / isolation & purification*
Cell Cycle Proteins / immunology
Cell Cycle Proteins / isolation & purification*
Enzyme-Linked Immunosorbent Assay
HeLa Cells
Humans
Hybrid Cells
Mice
Microscopy, Immunoelectron
Microvilli / immunology
Mitosis / immunology*
Molecular Sequence Data
Spleen / cytology
|
| IF |
1.723
|
| Times Cited |
9
|
|
WOS Category
|
CELL BIOLOGY
DEVELOPMENTAL BIOLOGY
|
| Resource |
| Human and Animal Cells |
HeLa(RCB0007)
SP2/0-Ag14(RCB0209) |
