論文 - 詳細
| RRC ID | 39454 |
|---|---|
| 著者 | Ohta S, Muramatsu H, Senda T, Zou K, Iwata H, Muramatsu T. |
| タイトル | Midkine is expressed during repair of bone fracture and promotes chondrogenesis. |
| ジャーナル | J Bone Miner Res |
| Abstract |
Midkine (MK) is a heparin-binding growth/differentiation factor implicated in the control of development and repair of various tissues. Upon fracture of the murine tibia, MK was found to be transiently expressed during bone repair. MK was immunohistochemically detected in spindle-shaped mesenchymal cells at the fracture site on day 4 after fracture and in chondrocytes in the area of endochondral ossification on day 7. MK expression was decreased on day 14 and scarcely seen on day 28 when bone repair was completed. This mode of MK expression is reminiscent of MK expression during development. MK was expressed in hypertrophic chondrocytes of the prebone cartilage rudiments on embryonic day 14 in mouse embryos. MK was also strongly expressed in the epiphyseal growth plate. MK was localized intracellularly during both bone repair and development, and this localization was confirmed by immunoelectron microscopy for embryonic chondrocytes. When MK cDNA was transfected into ATDC5 chondrogenic cells and overexpressed, the majority of transfected cells with strong MK expression showed enhanced chondrogenesis as revealed by increased synthesis of sulfated glycosaminoglycans, aggrecan, and type II collagen. These results suggest that MK plays important roles in chondrogenesis and contributes to bone formation and repair. |
| 巻・号 | 14(7) |
| ページ | 1132-44 |
| 公開日 | 1999-7-1 |
| DOI | 10.1359/jbmr.1999.14.7.1132 |
| PII | jbm297 |
| PMID | 10404013 |
| MeSH | Aggrecans Animals Animals, Newborn Blotting, Northern Carrier Proteins / biosynthesis* Carrier Proteins / genetics Cell Line Chondrocytes / metabolism Chondrocytes / ultrastructure Chondrogenesis / genetics Chondrogenesis / physiology* Cytokines / biosynthesis Cytokines / genetics DNA, Complementary / genetics Embryo, Mammalian Extracellular Matrix Proteins* Fracture Healing / physiology* Growth Plate / cytology Growth Plate / metabolism Immunohistochemistry Lectins, C-Type Limb Buds / metabolism Limb Buds / ultrastructure Mice Mice, Inbred ICR Microscopy, Immunoelectron Midkine Osteogenesis / genetics Osteogenesis / physiology* Proteoglycans / biosynthesis Transfection |
| IF | 5.854 |
| 引用数 | 47 |
| WOS 分野 | ENDOCRINOLOGY & METABOLISM |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | ATDC5(RCB0565) |