論文 - 詳細
| RRC ID | 39475 |
|---|---|
| 著者 | Haraguchi T, Ding DQ, Yamamoto A, Kaneda T, Koujin T, Hiraoka Y. |
| タイトル | Multiple-color fluorescence imaging of chromosomes and microtubules in living cells. |
| ジャーナル | Cell Struct Funct |
| Abstract |
Microscopic observation of fluorescently-stained intracellular molecules within a living cell provides a straightforward approach to understanding their temporal and spatial relationships. However, exposure to the excitation light used to visualize these fluorescently-stained molecules can be toxic to the cells. Here we describe several important considerations in microscope instrumentation and experimental conditions for avoiding the toxicity associated with observing living fluorescently-stained cells. Using a computer-controlled fluorescence microscope system designed for live observation, we recorded time-lapse, multi-color images of chromosomes and microtubules in living human and fission yeast cells. In HeLa cells, a human cell line, microtubules were stained with rhodamine-conjugated tubulin, and chromosomes were stained with a DNA-specific fluorescent dye, Hoechst33342, or with rhodamine-conjugated histone. In fission yeast cells, microtubules were stained with alpha-tubulin fused with the jellyfish green fluorescent protein (GFP), and chromosomes were stained with Hoechst33342. |
| 巻・号 | 24(5) |
| ページ | 291-8 |
| 公開日 | 1999-10-1 |
| DOI | 10.1247/csf.24.291 |
| PMID | 15216885 |
| MeSH | Benzimidazoles / chemistry Cell Cycle / physiology Cell Division / physiology Chromosomes / chemistry Chromosomes / metabolism* Chromosomes, Fungal / chemistry Chromosomes, Fungal / metabolism Chromosomes, Human / chemistry Chromosomes, Human / metabolism DNA / chemistry Fluorescent Dyes / chemistry Green Fluorescent Proteins HeLa Cells Histones / chemistry Histones / genetics Humans Indoles / chemistry Luminescent Proteins / chemistry Luminescent Proteins / genetics Microscopy, Fluorescence / instrumentation Microscopy, Fluorescence / methods Microtubules / chemistry Microtubules / metabolism* Mitosis / physiology Rhodamines / chemistry Schizosaccharomyces / genetics Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / genetics Thiamine / pharmacology Tubulin / chemistry Tubulin / genetics |
| IF | 2.429 |
| 引用数 | 78 |
| WOS 分野 | CELL BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |