| RRC ID |
39483
|
| 著者 |
Nakagawa I, Nakata M, Kawabata S, Hamada S.
|
| タイトル |
Regulated expression of the Shiga toxin B gene induces apoptosis in mammalian fibroblastic cells.
|
| ジャーナル |
Mol Microbiol
|
| Abstract |
Shiga toxins (Stxs) produced by enterohaemorrhagic Escherichia coli may induce colonic ulceration, bloody diarrhoea and acute renal failure. The A subunit (StxA) is known to inhibit protein synthesis, whereas the B subunits (StxB) bind to Gb3 on the cell surface. However, the mechanisms by which Stxs kill target cells remain unclear. Stx1A or Stx1B genes were introduced into pcDNA3.1 vectors and transfected into NIH3T3 and HeLa cells. The Stx1B gene-transfected cells became apoptotic with accompanying DNA fragmentation, whereas the Stx1A gene-transfected cells were found to be necrotic and no DNA fragmentation occurred. The HeLa/C4 cells integrated with the Stx1B gene with a tetracycline-inducible promoter eventually produced cytoplasmic Stx1B, leading to DNA fragmentation on the addition of doxycycline. These apoptotic changes were abrogated by pretreatment with Z-VAD-fmk. These results suggest that the transfected Stx1B gene induces apoptosis by activating the caspase cascade after Stx1B expression in the cytoplasm.
|
| 巻・号 |
33(6)
|
| ページ |
1190-9
|
| 公開日 |
1999-9-1
|
| DOI |
10.1046/j.1365-2958.1999.01564.x
|
| PII |
mmi1564
|
| PMID |
10510233
|
| MeSH |
3T3 Cells
Animals
Apoptosis* / drug effects
Bacterial Toxins / genetics*
Bacterial Toxins / toxicity
Base Sequence
Caspases / metabolism
DNA Fragmentation
DNA Primers / genetics
Escherichia coli O157 / genetics*
Escherichia coli O157 / pathogenicity
Gene Expression Regulation, Bacterial
Genes, Bacterial*
HeLa Cells
Humans
Interleukin-1 / biosynthesis
Mice
Plasmids / genetics
Shiga Toxins
Transfection
Tumor Necrosis Factor-alpha / biosynthesis
|
| IF |
3.418
|
| 引用数 |
42
|
|
WOS 分野
|
MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
NIH 3T3-3(RCB0150)
HeLa(RCB0007) |
