論文 - 詳細
| RRC ID | 39485 |
|---|---|
| 著者 | Yoshida A, Minowa MT, Takamatsu S, Hara T, Oguri S, Ikenaga H, Takeuchi M. |
| タイトル | Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase. |
| ジャーナル | Glycobiology |
| Abstract |
A human cDNA for UDP- N -acetylglucosamine:alpha1,3-d-mannoside beta1,4- N- acetylglucosaminyltransferase (GnT-IV) was isolated from a liver cDNA library using a probe based on a partial cDNA sequence of the bovine GnT-IV. The cDNA encoded a complete sequence of a type II membrane protein of 535 amino acids which is 96% identical to the bovine GnT-IV. Transient expression of the human cDNA in COS7 cells increased total cellular GnT-IV activity 25-fold, demonstrating that this cDNA encodes a functional human GnT-IV. Northern blot analysis of normal tissues indicated that at least five different sizes of mRNA (9.7, 7.6, 5.1, 3.8, and 2.4 kb) forGnT-IV are expressed in vivo. Furthermore, these mRNAs are expressed at different levels between tissues. Large amounts of mRNA were detected in tissues harboring T lineage cells. Also, the promyelocytic leukemia cell line HL-60 and the lymphoblastic leukemia cell line MOLT-4 revealed abundant mRNA. Lastly, the gene was mapped at the locus on human chromosome 2, band q12 by fluorescent in situ hybridization. |
| 巻・号 | 9(3) |
| ページ | 303-10 |
| 公開日 | 1999-3-1 |
| DOI | 10.1093/glycob/9.3.303 |
| PII | cwc029 |
| PMID | 10024668 |
| MeSH | Amino Acid Sequence Animals Base Sequence Blotting, Northern COS Cells Cell Lineage Chromosome Mapping Chromosomes, Human, Pair 2 / genetics* DNA, Complementary / genetics Gene Expression Humans In Situ Hybridization, Fluorescence Leukemia / enzymology Lymphoid Tissue / enzymology Membrane Proteins / genetics* Molecular Sequence Data N-Acetylglucosaminyltransferases / genetics* Neoplasm Proteins / genetics RNA, Messenger / isolation & purification Recombinant Proteins / biosynthesis T-Lymphocytes / enzymology* Tissue Distribution Tumor Cells, Cultured |
| IF | 4.06 |
| 引用数 | 21 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 12 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | COS-7(RCB0539) |