論文 - 詳細
| RRC ID | 39494 |
|---|---|
| 著者 | Okada S, Yoshida T, Hong Z, Ishii G, Hatano M, Kuro-O M, Nabeshima Y, Nabeshima Y, Tokuhisa T. |
| タイトル | Impairment of B lymphopoiesis in precocious aging (klotho) mice. |
| ジャーナル | Int Immunol |
| Abstract |
Inactivation of the klotho gene in mice results in multiple disorders that resemble human aging after 3 weeks of age. Because hematopoiesis, especially B lymphopoiesis, is affected in humans and mice by aging, we analyzed the hematopoietic state in homozygous klotho (kl/kl) mice. The kl/kl mice showed thymic atrophy and a reduced number of splenocytes. These mice had almost the normal number of myeloid cells, erythroid cells, IL-3-responsive myeloid precursors and colony forming units in spleen (CFU-S) in bone marrow (BM), but had a substantially decreased number of B cells in BM and peripheral blood as compared with wild-type mice. IL-7-responsive B cell precursors and all of the maturation stages of B cells in BM were also reduced. However, the function of hematopoietic stem cells including their capacity of B lymphopoiesis in vivo and in vitro was normal. Early B cell development was also normal in neonates and young kl/kl mice until 2 weeks old without aging phenotypes. RT-PCR analysis revealed that the level of IL-7 gene expression was significantly reduced in freshly isolated kl/kl BM cells. However, injection of IL-7 in kl/kl mice could not rescue the B lymphopenia. These findings indicate that Klotho protein may regulate B lymphopoiesis via its influence on the hematopoietic microenvironment. |
| 巻・号 | 12(6) |
| ページ | 861-71 |
| 公開日 | 2000-6-1 |
| DOI | 10.1093/intimm/12.6.861 |
| PMID | 10837414 |
| MeSH | Aging / immunology* Animals B-Lymphocytes / physiology* Bone Marrow Cells / physiology Hematopoiesis* Hematopoietic Stem Cells / physiology Interleukin-7 / biosynthesis Mice Mice, Inbred C3H Mice, Mutant Strains |
| IF | 3.519 |
| 引用数 | 42 |
| WOS 分野 | IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | OP9(RCB1124) |