論文 - 詳細
| RRC ID | 39513 |
|---|---|
| 著者 | Ko SH, Rho da J, Jeon JI, Kim YJ, Woo HA, Lee YK, Kim JM. |
| タイトル | Bacteroides fragilis Enterotoxin Upregulates Heme Oxygenase-1 in Intestinal Epithelial Cells via a Mitogen-Activated Protein Kinase- and NF-κB-Dependent Pathway, Leading to Modulation of Apoptosis. |
| ジャーナル | Infect Immun |
| Abstract |
The Bacteroides fragilis enterotoxin (BFT), a virulence factor of enterotoxigenic B. fragilis (ETBF), interacts with intestinal epithelial cells and can provoke signals that induce mucosal inflammation. Although expression of heme oxygenase-1 (HO-1) is associated with regulation of inflammatory responses, little is known about HO-1 induction in ETBF infection. This study was conducted to investigate the effect of BFT on HO-1 expression in intestinal epithelial cells. Stimulation of intestinal epithelial cells with BFT resulted in upregulated expression of HO-1. BFT activated transcription factors such as NF-κB, AP-1, and Nrf2 in intestinal epithelial cells. Upregulation of HO-1 in intestinal epithelial cells was dependent on activated IκB kinase (IKK)-NF-κB signals. However, suppression of Nrf2 or AP-1 signals in intestinal epithelial cells did not result in significant attenuation of BFT-induced HO-1 expression. HO-1 induction via IKK-NF-κB in intestinal epithelial cells was regulated by p38 mitogen-activated protein kinases (MAPKs). Furthermore, suppression of HO-1 activity led to increased apoptosis in BFT-stimulated epithelial cells. These results suggest that a signaling pathway involving p38 MAPK-IKK-NF-κB in intestinal epithelial cells is required for HO-1 induction during exposure to BFT. Following this induction, increased HO-1 expression may regulate the apoptotic process in responses to BFT stimulation. |
| 巻・号 | 84(9) |
| ページ | 2541-54 |
| 公開日 | 2016-9-1 |
| DOI | 10.1128/IAI.00191-16 |
| PII | IAI.00191-16 |
| PMID | 27324483 |
| PMC | PMC4995915 |
| MeSH | Animals Apoptosis / immunology* Bacteroides fragilis / immunology* Enterotoxins / immunology* Epithelial Cells / immunology Epithelial Cells / metabolism Epithelial Cells / microbiology* Heme Oxygenase-1 / metabolism* I-kappa B Proteins / metabolism Intestinal Mucosa / metabolism Intestinal Mucosa / microbiology* Mice Mice, Inbred C57BL Mice, Knockout Mitogen-Activated Protein Kinases / metabolism* NF-E2-Related Factor 2 / metabolism NF-kappa B / metabolism* Signal Transduction / immunology Transcription Factor AP-1 / metabolism Transcriptional Activation / immunology Up-Regulation / immunology p38 Mitogen-Activated Protein Kinases / metabolism |
| IF | 3.201 |
| 引用数 | 9 |
| WOS 分野 | INFECTIOUS DISEASES IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC01390 |