RRC ID 39516
Author Kowalska A, Siwicki AK, Kowalski RK.
Title Dietary resveratrol improves immunity but reduces reproduction of broodstock medaka Oryzias latipes (Temminck & Schlegel).
Journal Fish Physiol. Biochem.
Abstract Here, we investigated the effect of dietary resveratrol (20, 40, and 80 µg/g BW/day) on cell-mediated immunity (activity of spleen phagocytes and proliferative response of lymphocytes) and reproductive parameters (egg and sperm quality, i.e. fecundity-total number of eggs produced by individual fish, fertility, embryo survival, and hatching rate) in medaka. Fish fed feed with resveratrol at 40 and 80 µg/g BW/day had significantly higher metabolic activity and intracellular phagocyte killing activity than control. The proliferative lymphocyte activity of the fish from R80 group was greater by more than 20 % in comparison with the control group (P < 0.05). The percentage of macrophages (MO) and their mean fluorescence intensities (MFI) in R40 and R80 groups were significantly higher compared to C and R20 groups (P < 0.05). The differences in MO and MFI values ranged from 52.5 % (±1.5; R0 group) to 65.8 % (±1.6; R80 group) and from 23.2 (±1.4; R0 group) to 38.2 (±2.4; R80 group), respectively. Moreover, resveratrol at 80 µg/g BW/day decreased liver COX activity, i.e. 5.4 in R80 group and 7.9 in R0 group (P < 0.05). The motility parameters of the sperm obtained from the males fed feed supplemented with resveratrol at 80 µg/g BW/day exhibited the highest values except the linearity, which was lower as compared to the control (P < 0.05). The results indicate that diet supplemented with resveratrol at a dosage of 40 µg/g BW/day improves phagocyte killing ability and lymphocyte proliferation in broodstock and accelerates offspring hatch. Also, the results suggest that COX activity influences sperm and oocyte quality in fish; the presence of a COX inhibitor in the dose of 40 µg/g BW/day decreased the embryo survival.
Volume 43(1)
Pages 27-37
Published 2017-2
DOI 10.1007/s10695-016-0265-8
PII 10.1007/s10695-016-0265-8
PMID 27431678
PMC PMC5306266
MeSH Animals Cell Proliferation / drug effects Diet / veterinary* Female Immunity, Cellular / drug effects Immunologic Factors / pharmacology* Liver / drug effects Liver / enzymology Lymphocytes / drug effects Lymphocytes / physiology Macrophages / drug effects Macrophages / immunology Male Oryzias / immunology* Oryzias / physiology* Phagocytosis / drug effects Prostaglandin-Endoperoxide Synthases / metabolism Reproduction / drug effects* Sperm Motility / drug effects Spleen / cytology Stilbenes / pharmacology*
IF 1.735
Times Cited 1
WOS Category FISHERIES PHYSIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Medaka HdrR-II1(IB178)