論文 - 詳細
RRC ID | 39529 |
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著者 | Ogata S, Okumura K, Taguchi H. |
タイトル | A simple and rapid method for the detection of poly(ADP-ribose) by flow cytometry. |
ジャーナル | Biosci Biotechnol Biochem |
Abstract |
Measurement of poly(ADP-ribose) levels was performed by a new method using a monoclonal antibody against poly(ADP-ribose) and flow cytometry from small amount of cultured cells without the need for isolation of poly(ADP-ribose) polymer. The increase of poly(ADP-ribose)-associated fluorescence intensity was observed in individual human leukemic HL-60 cells when treated with the carcinogen, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), and was blocked by the treatment with 3-aminobenzamide before MNNG treatment. It is easy and rapid to detect the time-dependent change of poly(ADP-ribose) levels in HL-60 cells after MNNG treatment. We easily found that the increase of the poly(ADP-ribose) level in nicotinic acid-treated lymphocytes after MNNG treatment was observed, but not in nicotinamide-treated lymphocytes. We investigated the change of poly(ADP-ribose) levels especially in the early phase of apoptosis. Our method is simple and rapid. It is suggested that the investigation of poly(ADP-ribosyl)ation in various fields is possible by using this new method. |
巻・号 | 64(3) |
ページ | 510-5 |
公開日 | 2000-3-1 |
DOI | 10.1271/bbb.64.510 |
PMID | 10803947 |
MeSH | Benzamides / pharmacology Enzyme Inhibitors / pharmacology Flow Cytometry / methods* HL-60 Cells Humans Methylnitronitrosoguanidine / pharmacology Poly Adenosine Diphosphate Ribose / analysis* Poly(ADP-ribose) Polymerase Inhibitors |
IF | 1.516 |
引用数 | 7 |
WOS 分野 | CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
リソース情報 | |
ヒト・動物細胞 |