Reference - Detail
|Author||Ogata S, Okumura K, Taguchi H.|
|Title||A simple and rapid method for the detection of poly(ADP-ribose) by flow cytometry.|
|Journal||Biosci Biotechnol Biochem|
Measurement of poly(ADP-ribose) levels was performed by a new method using a monoclonal antibody against poly(ADP-ribose) and flow cytometry from small amount of cultured cells without the need for isolation of poly(ADP-ribose) polymer. The increase of poly(ADP-ribose)-associated fluorescence intensity was observed in individual human leukemic HL-60 cells when treated with the carcinogen, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), and was blocked by the treatment with 3-aminobenzamide before MNNG treatment. It is easy and rapid to detect the time-dependent change of poly(ADP-ribose) levels in HL-60 cells after MNNG treatment. We easily found that the increase of the poly(ADP-ribose) level in nicotinic acid-treated lymphocytes after MNNG treatment was observed, but not in nicotinamide-treated lymphocytes. We investigated the change of poly(ADP-ribose) levels especially in the early phase of apoptosis. Our method is simple and rapid. It is suggested that the investigation of poly(ADP-ribosyl)ation in various fields is possible by using this new method.
|MeSH||Benzamides / pharmacology Enzyme Inhibitors / pharmacology Flow Cytometry / methods* HL-60 Cells Humans Methylnitronitrosoguanidine / pharmacology Poly Adenosine Diphosphate Ribose / analysis* Poly(ADP-ribose) Polymerase Inhibitors|
|WOS Category||CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY|
|Human and Animal Cells|