RRC ID 39529
Author Ogata S, Okumura K, Taguchi H.
Title A simple and rapid method for the detection of poly(ADP-ribose) by flow cytometry.
Journal Biosci Biotechnol Biochem
Abstract Measurement of poly(ADP-ribose) levels was performed by a new method using a monoclonal antibody against poly(ADP-ribose) and flow cytometry from small amount of cultured cells without the need for isolation of poly(ADP-ribose) polymer. The increase of poly(ADP-ribose)-associated fluorescence intensity was observed in individual human leukemic HL-60 cells when treated with the carcinogen, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), and was blocked by the treatment with 3-aminobenzamide before MNNG treatment. It is easy and rapid to detect the time-dependent change of poly(ADP-ribose) levels in HL-60 cells after MNNG treatment. We easily found that the increase of the poly(ADP-ribose) level in nicotinic acid-treated lymphocytes after MNNG treatment was observed, but not in nicotinamide-treated lymphocytes. We investigated the change of poly(ADP-ribose) levels especially in the early phase of apoptosis. Our method is simple and rapid. It is suggested that the investigation of poly(ADP-ribosyl)ation in various fields is possible by using this new method.
Volume 64(3)
Pages 510-5
Published 2000-3
DOI 10.1271/bbb.64.510
PMID 10803947
MeSH Benzamides / pharmacology Enzyme Inhibitors / pharmacology Flow Cytometry / methods* HL-60 Cells Humans Methylnitronitrosoguanidine / pharmacology Poly Adenosine Diphosphate Ribose / analysis* Poly(ADP-ribose) Polymerase Inhibitors
IF 1.297
Times Cited 7
WOS Category CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Human and Animal Cells