| RRC ID |
39540
|
| Author |
Mashima J, Nagahama M, Hatsuzawa K, Tani K, Horigome T, Yamamoto A, Tagaya M.
|
| Title |
N-ethylmaleimide-sensitive factor is associated with the nuclear envelope.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
N-Ethylmaleimide-sensitive factor (NSF) is an ATPase involved in many membrane fusion events within the exocytic and endocytotic pathways. In the present study we showed that NSF is associated with the nuclear envelope. Golgi-associated NSF was released from membranes upon incubation with Mg(2+)-ATP, reflecting the disassembly of a complex consisting of NSF, soluble NSF attachment proteins (SNAPs), and SNAP receptors (SNAREs). In contrast nuclear envelope-associated NSF in interphase cells was not released by the same treatment. During mitosis, however, it was released from nuclear membranes by Mg(2+)-ATP. These results suggest that the binding mode of nuclear membrane-associated NSF changes during the cell cycle.
|
| Volume |
274(2)
|
| Pages |
559-64
|
| Published |
2000-8-2
|
| DOI |
10.1006/bbrc.2000.3162
|
| PII |
S0006-291X(00)93162-0
|
| PMID |
10913377
|
| MeSH |
Adenosine Triphosphate / pharmacology
Animals
Carrier Proteins / analysis
Carrier Proteins / metabolism*
Cell Cycle
Cell Division
Cell Fractionation
Cell Nucleus / chemistry
Cell Nucleus / metabolism
Cytoplasm / metabolism
Immunoblotting
Interphase
Lamins
Mannosidases / analysis
Mitosis / drug effects
N-Ethylmaleimide-Sensitive Proteins
Nuclear Envelope / metabolism*
Nuclear Envelope / ultrastructure
Nuclear Proteins / metabolism
PC12 Cells
Rats
Vesicular Transport Proteins*
|
| IF |
2.985
|
| Times Cited |
3
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
PC-12(RCB0009)
NRK(RCB0043) |
