Reference - Detail
| RRC ID | 39545 |
|---|---|
| Author | Kinpara K, Mogi M, Kuzushima M, Togari A. |
| Title | Osteoclast differentiation factor in human osteosarcoma cell line. |
| Journal | J Immunoassay |
| Abstract |
A sensitive sandwich enzyme-linked immunosorbent assay (ELISA) for human osteoclast differentiation factor (ODF/RANKL/ OPGL/TRANCE) utilizing a polyclonal antibody that recognizes both human soluble ODF and mouse ODF in combination with a osteoclasogenesis inhibitory factor (OCIF/OPG) was developed. We can quantify the ODF level in not only human ODF (detection limit: 0.05 ng/ml), but also mouse ODF by virtue of cross-reactivity. Employing this assay system, we demonstrated that ODF is constitutively present as a membrane-bound form in both the human osteosarcoma cell lines, MG-63, HOS and SaOS-2, and the mouse osteoblastic cell line MC3T3-E1. |
| Volume | 21(4) |
| Pages | 327-40 |
| Published | 2000-11-1 |
| DOI | 10.1080/01971520009349540 |
| PMID | 11071251 |
| MeSH | Animals Carrier Proteins / analysis* Carrier Proteins / metabolism Cell Differentiation Cross Reactions Enzyme-Linked Immunosorbent Assay / methods* Glycoproteins / metabolism Humans Membrane Glycoproteins / analysis* Membrane Glycoproteins / metabolism Mice Osteoblasts / cytology Osteoclasts / cytology* Osteoprotegerin Osteosarcoma Protein Binding RANK Ligand Receptor Activator of Nuclear Factor-kappa B Receptors, Cytoplasmic and Nuclear / metabolism Receptors, Tumor Necrosis Factor Tumor Cells, Cultured |
| Times Cited | 29 |
| WOS Category | MEDICAL LABORATORY TECHNOLOGY IMMUNOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Human and Animal Cells | Saos-2(RCB0428) |