論文 - 詳細
| RRC ID | 39580 |
|---|---|
| 著者 | Omasa T, Cao Y, Park JY, Takagi Y, Kimura S, Yano H, Honda K, Asakawa S, Shimizu N, Ohtake H. |
| タイトル | Bacterial artificial chromosome library for genome-wide analysis of Chinese hamster ovary cells. |
| ジャーナル | Biotechnol Bioeng |
| Abstract |
Chinese hamster ovary (CHO) cell lines are widely used for scientific research and biotechnology. A CHO genomic bacterial artificial chromosome (BAC) library was constructed from a mouse dihydrofolate reductase (DHFR) gene-amplified CHO DR1000L-4N cell line for genome-wide analysis of CHO cell lines. The CHO BAC library consisted of 122,281 clones and was expected to cover the entire CHO genome five times. A CHO chromosomal map was constructed by fluorescence in situ hybridization (FISH) imaging using BAC clones as hybridization probes (BAC-FISH). Thirteen BAC-FISH marker clones were necessary to identify all the 20 individual chromosomes in a DHFR-deficient CHO DG44 cell line because of the aneuploidy of the cell line. To determine the genomic structure of the exogenous Dhfr amplicon, a 165-kb DNA region containing exogenous Dhfr was cloned from the BAC library using high-density replica (HDR) filters and Southern blot analysis. The nucleotide sequence analysis revealed a novel genomic structure in which the vector sequence containing Dhfr was sandwiched by long inverted sequences of the CHO genome. |
| 巻・号 | 104(5) |
| ページ | 986-94 |
| 公開日 | 2009-12-1 |
| DOI | 10.1002/bit.22463 |
| PMID | 19575438 |
| MeSH | Animals CHO Cells* Chromosome Mapping Chromosomes, Artificial, Bacterial* Cricetinae Cricetulus Gene Library* Genetic Vectors Genome* In Situ Hybridization, Fluorescence Mice Recombinant Proteins / genetics Tetrahydrofolate Dehydrogenase / genetics |
| IF | 4.002 |
| 引用数 | 23 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | Chinese hamster genomic BAC library clone (RDB13444) |