RRC ID 39587
Author Fukuda M, Saegusa C, Mikoshiba K.
Title Novel splicing isoforms of synaptotagmin-like proteins 2 and 3: identification of the Slp homology domain.
Journal Biochem Biophys Res Commun
Abstract Slp1-3 (synaptotagmin-like protein 1-3) is a new family of carboxyl-terminal-type (C-type) tandem C2 proteins that show higher sequence similarity to the C2 domains of granuphilin-a/Slp-4 than those of other C-type tandem C2 proteins (e.g., synaptotagmin and the Doc2 family). However, the amino (N)-terminal domains of the original Slp1-3 do not contain any known protein motifs and do not show any sequence similarities to each other. We report four alternative splicing isoforms of Slp2 (designated Slp2-a-d, with the original Slp2 renamed Slp2-c) and two alternative splicing isoforms of Slp3 (Slp3-a and Slp3-b, the original Slp3). These isoforms share the same C-terminal tandem C2 structures, but their N-terminal nucleotide sequences are completely different due to the alternate use of different exons. Sequence alignment of the Slp1, Slp2-a, Slp3-a, and Slp4 amino terminal domains reveals the presence of two conserved regions among the Slp family, designated SHD1 (Slp homology domain 1) and SHD2, which may function as protein interaction sites. The SHD1 and SHD2 of Slp3-a and Slp4 are separated by a putative Zn(2+)-binding sequence, whereas Slp1 and Slp2 lack such Zn(2+)-binding sequences and their SHD1 and SHD2 are linked together. In addition, we show that the Slp2-a/c/d mRNAs are differentially distributed in different mouse tissues and at different stages of development, suggesting that these transcripts may be regulated by different promoters.
Volume 283(2)
Pages 513-9
Published 2001-5-4
DOI 10.1006/bbrc.2001.4803
PII S0006-291X(01)94803-X
PMID 11327731
MeSH Alternative Splicing Amino Acid Sequence Animals Base Sequence Binding Sites Cloning, Molecular DNA Primers / genetics In Vitro Techniques Male Membrane Proteins / chemistry* Membrane Proteins / genetics* Membrane Proteins / metabolism Mice Molecular Sequence Data Protein Isoforms / chemistry Protein Isoforms / genetics Protein Isoforms / metabolism Protein Structure, Tertiary RNA, Messenger / genetics RNA, Messenger / metabolism Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Tissue Distribution Zinc / metabolism
IF 2.985
Times Cited 69
DNA material Slac2-a (RDB03422)