論文 - 詳細
| RRC ID | 39599 |
|---|---|
| 著者 | Ui-Tei K, Zenno S, Miyata Y, Saigo K. |
| タイトル | Sensitive assay of RNA interference in Drosophila and Chinese hamster cultured cells using firefly luciferase gene as target. |
| ジャーナル | FEBS Lett |
| Abstract |
A sensitive cellular assay system for RNA interference was developed using the firefly luciferase gene as target. RNA interference was noted not only in Drosophila cultured cells but Chinese hamster cells (CHO-K1) as well, although double-stranded RNA required for the latter was 2500 times more than for the former. Cognate double-stranded RNA as short as 38 bp was found to be still capable of inducing RNA interference in Drosophila cultured cells. |
| 巻・号 | 479(3) |
| ページ | 79-82 |
| 公開日 | 2000-8-18 |
| DOI | 10.1016/s0014-5793(00)01883-4 |
| PII | S0014-5793(00)01883-4 |
| PMID | 10981711 |
| MeSH | Animals CHO Cells Cell Culture Techniques / methods* Cell Line Cricetinae DNA / metabolism Dose-Response Relationship, Drug Drosophila Luciferases / genetics* Luciferases / metabolism Models, Genetic Molecular Sequence Data Plasmids RNA / analysis* RNA / metabolism RNA, Double-Stranded / metabolism Transfection |
| IF | 3.057 |
| 引用数 | 87 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 658 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 9.0 |
| リソース情報 | |
| ヒト・動物細胞 | CHO-K1(RCB0285) |