| RRC ID |
39623
|
| 著者 |
Murata-Hori M, Fumoto K, Fukuta Y, Iwasaki T, Kikuchi A, Tatsuka M, Hosoya H.
|
| タイトル |
Myosin II regulatory light chain as a novel substrate for AIM-1, an aurora/Ipl1p-related kinase from rat.
|
| ジャーナル |
J Biochem
|
| Abstract |
Previous studies demonstrated that the phosphorylated myosin II regulatory light chain (MRLC) is localized at the cleavage furrow of dividing cells, suggesting that phosphorylation of MRLC plays an important role in cytokinesis. However, it remains unclear which kinase(s) phosphorylate MRLC during cytokinesis. AIM-1, an Aurora/Ipl1p-related kinase from rat, is known as a serine/threonine kinase that is required for cytokinesis. Here we examined the possibility that AIM-1 is a candidate for a kinase that phosphorylates MRLC during cytokinesis. As a result, we showed that AIM-1 monophosphorylated MRLC at Ser19 using two-dimensional phosphopeptide mapping analysis and several MRLC mutants. Furthermore, AIM-1 was colocalized with monophosphorylated MRLC at the cleavage furrow of dividing cells. We propose here that AIM-1 may participate in monophosphorylation of MRLC during cytokinesis.
|
| 巻・号 |
128(6)
|
| ページ |
903-7
|
| 公開日 |
2000-12-1
|
| DOI |
10.1093/oxfordjournals.jbchem.a022840
|
| PMID |
11098131
|
| MeSH |
Animals
Aurora Kinases
Cell Line
Humans
Myosins / chemistry
Myosins / metabolism*
Peptide Mapping
Phosphorylation
Protein Kinases / metabolism*
Protein Serine-Threonine Kinases*
Rats
Substrate Specificity
|
| IF |
2.476
|
| 引用数 |
53
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
COS-7(RCB0539)
HeLa(RCB0007) |
