RRC ID 39635
著者 Takahashi F, Takahashi K, Maeda K, Tominaga S, Fukuchi Y.
タイトル Osteopontin is induced by nitric oxide in RAW 264.7 cells.
ジャーナル IUBMB Life
Abstract Nitric oxide (NO) produced by macrophages is thought to contribute to various pathological conditions. Osteopontin (OPN) is a phosphorylated glycoprotein produced principally by macrophages. OPN inhibits inducible nitric oxide synthase (iNOS), which generates large amounts of NO production. However, the relationship between NO and endogenous OPN in activated macrophages has not yet been elucidated. We therefore examined expression of endogenous iNOS and OPN in a murine macrophage cell line, RAW 264.7 cells, by treating the cells with lipopolysaccharide (LPS) and interferon-gamma (IFN-gamma). Treatment of cells with LPS and IFN-gamma resulted in an increase of iNOS mRNA to maximum at 12 h after stimulation. In contrast, OPN mRNA was induced more slowly than iNOS mRNA. Induction of both iNOS and OPN mRNA in RAW 264.7 cells was markedly suppressed by addition of the specific iNOS inhibitor S-2-aminoethyl isothiourea dihydrobromide. The NOS inhibitor NG-methyl-L-arginine also suppressed induction of OPN mRNA but hardly affected iNOS mRNA expression. The NO-releasing agent spermine-NONOate but not peroxynitrite enhanced induction of OPN mRNA. These results suggest that NO directly up-regulates the endogenous OPN in macrophages stimulated with LPS and IFN-gamma. This up-regulation of endogenous OPN may represent a negative feedback system acting to reduce iNOS expression.
巻・号 49(3)
ページ 217-21
公開日 2000-3-1
DOI 10.1080/713803614
PMID 10868913
MeSH Animals Blotting, Northern Cell Line Enzyme Inhibitors / pharmacology Humans Interferon-gamma / pharmacology Kinetics Lipopolysaccharides / pharmacology Macrophages / enzymology Macrophages / metabolism Mice Nitrates / pharmacology Nitric Oxide / pharmacology* Nitric Oxide Donors / pharmacology Nitric Oxide Synthase / metabolism Nitric Oxide Synthase Type II Nitrogen Oxides Osteopontin Oxidants / pharmacology RNA, Messenger / metabolism Sialoglycoproteins / metabolism* Spermine / analogs & derivatives Spermine / pharmacology Time Factors beta-Aminoethyl Isothiourea / pharmacology* omega-N-Methylarginine / pharmacology
IF 3.244
引用数 28
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
ヒト・動物細胞 RAW 264(RCB0535)