RRC ID 39636
Author Kimura Y, Okuda H.
Title Effects of naturally occurring stilbene glucosides from medicinal plants and wine, on tumour growth and lung metastasis in Lewis lung carcinoma-bearing mice.
Journal J Pharm Pharmacol
Abstract Stilbene glucosides are naturally occurring phytoalexins, found in a variety of medicinal plants. Among the stilbene derivatives, resveratrol 3-O-D-glucoside (piceid) is found in grapes and wine. We studied the effects of stilbene glucosides isolated from medicinal plants and grapes on tumour growth and lung metastasis in mice bearing highly metastatic Lewis lung carcinoma (LLC) tumours. We also studied the inhibitory effects of stilbene glucosides on differentiation of human umbilical vein endothelial cells (HUVECs) to form a capillary network. Tumour growth in the right hind paw and lung metastasis were inhibited by oral administration of the stilbene glucosides, piceid and 2,3,5,4'-tetrahydroxystilbene-2-O-D-glucoside for 33 consecutive days, in LLC-bearing mice. As the number of CD8+ and NK1.1+ T cells in the spleen was not affected, the inhibitory effects of these stilbene glucosides on tumour growth and lung metastasis could not be explained by natural killer or cytotoxic T lymphocyte activation. Piceid inhibited the DNA synthesis in LLC cells at a concentration of 1000 microM, but not at lower concentrations (10-100 microM). 2,3,5,4'-Tetra-hydroxystilbene-2-O-D-glucoside also inhibited DNA synthesis in LLC cells (IC50 81 microM). In addition, both stilbene glucosides inhibited the formation of capillary-like tube networks (angiogenesis) of HUVECs at concentrations of 100 to 1000 microM. We suggest that the antitumour and antimetastatic activity of the stilbene glucosides, piceid and 2,3,5,4'-tetrahydroxystilbene-2-O-D-glucoside, might be due to the inhibition of DNA synthesis in LLC cells and angiogenesis of HUVECs.
Volume 52(10)
Pages 1287-95
Published 2000-10-1
DOI 10.1211/0022357001777270
PMID 11092574
MeSH Animals Antineoplastic Agents / therapeutic use* Carcinoma, Lewis Lung / drug therapy* Carcinoma, Lewis Lung / secondary Cattle DNA / biosynthesis DNA / drug effects Drug Screening Assays, Antitumor Endothelium, Vascular / drug effects Endothelium, Vascular / metabolism Female Glucosides / pharmacology* Glucosides / therapeutic use* Humans Mice Mice, Inbred C57BL Organ Size / drug effects Phytotherapy Plants, Medicinal / therapeutic use Resveratrol Ribonucleotide Reductases / antagonists & inhibitors* Spleen / drug effects Spleen / metabolism Stilbenes / pharmacology* Stilbenes / therapeutic use* Swine T-Lymphocytes / drug effects T-Lymphocytes / metabolism Thymus Gland / drug effects Thymus Gland / metabolism Umbilical Veins Wine / analysis
IF 2.571
Times Cited 75
Human and Animal Cells