| RRC ID |
39647
|
| 著者 |
Tanaka M, Ito S, Kiuchi K.
|
| タイトル |
Novel alternative promoters of mouse glial cell line-derived neurotrophic factor gene.
|
| ジャーナル |
Biochim Biophys Acta
|
| Abstract |
We previously isolated cDNA and genomic DNA of the mouse glial cell line-derived neurotrophic factor (GDNF) gene and found that the gene consists of three exons. Recently, it was suggested that an alternative promoter exists within intron 1 of the human GDNF gene, but this has not been confirmed. Novel cDNA clones of the mouse GDNF gene were isolated by 5'-rapid amplification of cDNA ends from postnatal day-14 striatum. A novel exon, containing 351 nucleotides, exists between exon 1 and exon 3 (referred to as exon 2 in our previous report). Luciferase reporter assay showed that a core promoter for the novel exon 2 requires its 5'-untranslated region. Primer extension analysis and reverse transcription-PCR identified another novel transcript that starts 39 bp upstream of exon 3, and the core promoter activity exists within a region containing putative Sp1 sites. Although the core promoters for the novel exons are different from those previously identified, transcripts derived from each promoter coincidentally increased with interleukin-1beta or tumor necrosis factor-alpha stimulation. Gel retardation assays suggested that the NF-kappaB binding site in intron 1 would be involved in the cytokine response of the mouse GDNF gene.
|
| 巻・号 |
1494(1-2)
|
| ページ |
63-74
|
| 公開日 |
2000-11-15
|
| DOI |
10.1016/s0167-4781(00)00218-9
|
| PII |
S0167-4781(00)00218-9
|
| PMID |
11072069
|
| MeSH |
Animals
Astrocytes / drug effects
Astrocytes / metabolism*
Base Sequence
Blotting, Southern
Cloning, Molecular
DNA / genetics
DNA / metabolism
Exons / genetics
Gene Expression Regulation* / drug effects
Glial Cell Line-Derived Neurotrophic Factor
Interleukin-1 / pharmacology
Introns / genetics
Mice
Molecular Sequence Data
NF-kappa B / metabolism
Neostriatum / cytology
Neostriatum / metabolism
Nerve Growth Factors*
Nerve Tissue Proteins / genetics*
Neurons / drug effects
Neurons / metabolism
Promoter Regions, Genetic / genetics*
RNA, Messenger / analysis
RNA, Messenger / genetics
RNA, Messenger / metabolism
Transcription, Genetic / drug effects
Transcription, Genetic / genetics*
Tumor Cells, Cultured
|
| IF |
3.411
|
| 引用数 |
28
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
C-1300(RCB0283) |
