論文 - 詳細
| RRC ID | 39654 |
|---|---|
| 著者 | Tsuchiya H, Doki S, Takemoto M, Ikuta T, Higuchi T, Fukui K, Usuda Y, Tabuchi E, Nagatoishi S, Tsumoto K, Nishizawa T, Ito K, Dohmae N, Ishitani R, Nureki O. |
| タイトル | Structural basis for amino acid export by DMT superfamily transporter YddG. |
| ジャーナル | Nature |
| Abstract |
The drug/metabolite transporter (DMT) superfamily is a large group of membrane transporters ubiquitously found in eukaryotes, bacteria and archaea, and includes exporters for a remarkably wide range of substrates, such as toxic compounds and metabolites. YddG is a bacterial DMT protein that expels aromatic amino acids and exogenous toxic compounds, thereby contributing to cellular homeostasis. Here we present structural and functional analyses of YddG. Using liposome-based analyses, we show that Escherichia coli and Starkeya novella YddG export various amino acids. The crystal structure of S. novella YddG at 2.4 Å resolution reveals a new membrane transporter topology, with ten transmembrane segments in an outward-facing state. The overall structure is basket-shaped, with a large substrate-binding cavity at the centre of the molecule, and is composed of inverted structural repeats related by two-fold pseudo-symmetry. On the basis of this intramolecular symmetry, we propose a structural model for the inward-facing state and a mechanism of the conformational change for substrate transport, which we confirmed by biochemical analyses. These findings provide a structural basis for the mechanism of transport of DMT superfamily proteins. |
| 巻・号 | 534(7607) |
| ページ | 417-20 |
| 公開日 | 2016-6-16 |
| DOI | 10.1038/nature17991 |
| PII | nature17991 |
| PMID | 27281193 |
| MeSH | Alphaproteobacteria / chemistry Alphaproteobacteria / metabolism Amino Acid Transport Systems, Neutral / chemistry* Amino Acid Transport Systems, Neutral / metabolism* Amino Acids / metabolism* Biological Transport Crystallography, X-Ray Escherichia coli / chemistry Escherichia coli / metabolism Escherichia coli Proteins / chemistry* Escherichia coli Proteins / metabolism* Liposomes / chemistry Liposomes / metabolism Models, Molecular Protein Conformation Structure-Activity Relationship |
| IF | 42.779 |
| 引用数 | 21 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 46 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | Genomic DNA of Starkeya novella JCM 20403T (JGD08351) Genomic DNA of Escherichia coli JCM 20135 (JGD07547). |
| 一般微生物 | |