Reference - Detail
| RRC ID | 39659 |
|---|---|
| Author | Hara S, Kato T, Goto Y, Kubota S, Tamano M, Terao M, Takada S. |
| Title | Microinjection-based generation of mutant mice with a double mutation and a 0.5 Mb deletion in their genome by the CRISPR/Cas9 system. |
| Journal | J Reprod Dev |
| Abstract |
The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system is a useful tool for genome editing. In this study, using a microinjection-based CRISPR/Cas9 system, we efficiently generated mouse lines carrying mutations at the Irx3 and Irx5 loci, which are located in close proximity on a chromosome and are functionally redundant. During the generation of Irx3/Irx5 double mutant mice, a deletion of ~0.5 Mb between the Irx3 and Irx5 loci was unintentionally identified in 6 out of 27 living pups by PCR based genotyping analysis. This deletion was confirmed by DNA fluorescence in situ hybridization analysis of fibroblasts. These results indicate that the mutant mice with a deletion of at least 0.5 Mb in their genome can be generated by the CRISPR/Cas9 system through microinjection into fertilized eggs. Our findings expand the utility of the CRISPR/Cas9 system in production of disease model animals with large deletions. |
| Volume | 62(5) |
| Pages | 531-536 |
| Published | 2016-10-18 |
| DOI | 10.1262/jrd.2016-058 |
| PMID | 27396308 |
| PMC | PMC5081742 |
| MeSH | Alleles Animals CRISPR-Cas Systems* Codon Exons Female Fibroblasts / metabolism Gene Deletion* Genetic Vectors Genome Genotype Homeodomain Proteins / genetics In Situ Hybridization, Fluorescence Mice Mice, Inbred C57BL Mice, Inbred DBA Microinjections Mutation* Sequence Analysis, DNA Transcription Factors / genetics |
| IF | 1.735 |
| Times Cited | 9 |
| WOS Category | AGRICULTURE, DAIRY & ANIMAL SCIENCE REPRODUCTIVE BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | B6N BAC Mouse (RDB07573) B6Ng01-076D03 B6Ng01-096G05 B6Ng01-008H07 B6Ng01-289E22. |