| RRC ID |
396
|
| Author |
Kawakami A, Nojima Y, Toyoda A, Takahoko M, Satoh M, Tanaka H, Wada H, Masai I, Terasaki H, Sakaki Y, Takeda H, Okamoto H.
|
| Title |
The zebrafish-secreted matrix protein you/scube2 is implicated in long-range regulation of hedgehog signaling.
|
| Journal |
Curr Biol
|
| Abstract |
The Hedgehog (Hh) signal plays a pivotal role in induction of ventral neuronal and muscle cell types around the midline during vertebrate development [1]. We report that the gene disrupted in zebrafish you mutants, in which Hh signaling is impaired, encodes the secreted matrix protein Scube2. Consistently, epistasis analyses suggested that Scube2 functions upstream of Hh ligands or through a parallel pathway. In addition, overexpression analyses suggested that Scube2 is an essential, but a permissive, mediator of Hh signaling in zebrafish embryos. Surprisingly, the you gene is expressed in the dorsal neural tube, raising the possibility that Scube2 could indirectly act via a long-range regulator of Hh signaling. The dorsal Bmps have a long-range and opposing influence on Hh signaling [2-5]. We show that neural plate patterning is affected in you mutants in a way that is consistent with the aberrant long-range action of a Bmp-dependent signal. We further show that Bmp activity can be attenuated by the coexpression of Scube2. Our data support the idea that Scube2 can modulate the long-range action of Bmp-dependent signaling in the neural tube and somites.
|
| Volume |
15(5)
|
| Pages |
480-8
|
| Published |
2005-3-8
|
| DOI |
10.1016/j.cub.2005.02.018
|
| PII |
S0960-9822(05)00157-0
|
| PMID |
15753045
|
| MeSH |
Amino Acid Sequence
Animals
Base Sequence
Blotting, Western
Body Patterning / physiology*
Bone Morphogenetic Proteins / metabolism
Central Nervous System / embryology*
Chromosome Mapping
DNA Primers
Epistasis, Genetic
Extracellular Matrix Proteins / genetics
Extracellular Matrix Proteins / metabolism*
Gene Components
Genotype
Hedgehog Proteins
In Situ Hybridization
Molecular Sequence Data
Mutation / genetics
Phenotype*
Sequence Alignment
Sequence Analysis, DNA
Signal Transduction / physiology*
Trans-Activators / metabolism*
Zebrafish / embryology
Zebrafish / metabolism*
Zebrafish Proteins / genetics
Zebrafish Proteins / metabolism*
|
| IF |
9.601
|
| Times Cited |
81
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| Zebrafish |
rw87
RIKEN WT (RW) |
