| RRC ID |
39728
|
| 著者 |
Takahashi T, Nishida T, Tuji A, Saito C, Matsuzaki R, Sato M, Toyooka K, Yasuda H, Nozaki H.
|
| タイトル |
Delineation of six species of the primitive algal genus Glaucocystis based on in situ ultrastructural characteristics.
|
| ジャーナル |
Sci Rep
|
| Abstract |
The field of microbiology was established in the 17(th) century upon the discovery of microorganisms by Antonie van Leeuwenhoek using a single-lens microscope. Now, the detailed ultrastructures of microorganisms can be elucidated in situ using three-dimensional electron microscopy. Since the availability of electron microscopy, the taxonomy of microscopic organisms has entered a new era. Here, we established a new taxonomic system of the primitive algal genus Glaucocystis (Glaucophyta) using a new-generation electron microscopic methodology: ultra-high-voltage electron microscopy (UHVEM) and field-emission scanning electron microscopy (FE-SEM). Various globally distributed Glaucocystis strains were delineated into six species, based on differences in in situ ultrastructural features of the protoplast periphery under UHVEM tomography and in the mother cell wall by FE-SEM, as well as differences in the light microscopic characteristics and molecular phylogenetic results. The present work on Glaucocystis provides a model case of new-generation taxonomy.
|
| 巻・号 |
6
|
| ページ |
29209
|
| 公開日 |
2016-7-7
|
| DOI |
10.1038/srep29209
|
| PII |
srep29209
|
| PMID |
27383831
|
| PMC |
PMC4935853
|
| MeSH |
DNA, Intergenic / chemistry
DNA, Intergenic / genetics
Glaucophyta / anatomy & histology
Glaucophyta / classification*
Glaucophyta / genetics
Glaucophyta / ultrastructure*
Nucleic Acid Conformation
Phylogeny*
Species Specificity
Tomography
|
| IF |
3.998
|
| 引用数 |
6
|
|
WOS 分野
|
MULTIDISCIPLINARY SCIENCES
|
| リソース情報 |
| 藻類 |
NIES-1961
NIES-2141
NIES-1369
NIES-966 |
