論文 - 詳細
| RRC ID | 39763 |
|---|---|
| 著者 | Kobayashi S, Sakae K, Suzuki Y, Shinozaki K, Okada M, Ishiko H, Kamata K, Suzuki K, Natori K, Miyamura T, Takeda N. |
| タイトル | Molecular cloning, expression, and antigenicity of Seto virus belonging to genogroup I Norwalk-like viruses. |
| ジャーナル | J Clin Microbiol |
| Abstract |
The viral capsid protein of the Seto virus (SeV), a Japanese strain of genogroup I Norwalk-like viruses (NLVs), was expressed as virus-like particles using a baculovirus expression system. An antigen detection enzyme-linked immunosorbent assay based on hyperimmune antisera to recombinant SeV was highly specific to homologous SeV-like strains but not heterologous strains in stools, allowing us type-specific detection of NLVs. |
| 巻・号 | 38(9) |
| ページ | 3492-4 |
| 公開日 | 2000-9-1 |
| DOI | 10.1128/JCM.38.9.3492-3494.2000 |
| PMID | 10970413 |
| PMC | PMC87416 |
| MeSH | Animals Antigens, Viral / analysis Blotting, Southern Caliciviridae / classification* Caliciviridae / genetics Caliciviridae / immunology* Caliciviridae Infections / virology* Capsid / genetics* Capsid / metabolism Cloning, Molecular Enzyme-Linked Immunosorbent Assay / methods Feces / virology Gastroenteritis / virology* Humans Molecular Sequence Data Norwalk virus / classification Norwalk virus / genetics Norwalk virus / immunology Rabbits Recombinant Proteins / immunology Reverse Transcriptase Polymerase Chain Reaction |
| IF | 5.897 |
| 引用数 | 28 |
| WOS 分野 | MICROBIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | Sf9(RCB0563) |