RRC ID 39780
著者 Tanaka T, Umesaki N.
タイトル Cytokine regulation of apoptotic susceptibility in a human endometrial epithelial cell line.
ジャーナル J Reprod Immunol
Abstract The effects of four endometrial cytokines, transforming growth factor beta1 (TGF-beta1), interleukin-1beta (IL-1beta), epidermal growth factor (EGF), and hepatocyte growth factor (HGF), on Fas-mediated apoptosis in the human endometrial epithelial cell line, HHUA, were investigated. Although the cell growth of HHUA was not directly affected by TGF-beta1, IL-1beta, or EGF, pretreatment of HHUA with TGF-beta1, IL-1beta, or EGF enhanced Fas-mediated growth suppression and Fas-mediated DNA fragmentation in the cells. Flow cytometric analyses demonstrated that TGF-beta1, IL-1beta, and EGF did not induce Fas expression on the cell surface. These results suggest that TGF-beta1, IL-1beta, and EGF enhances apoptotic susceptibility of the cells. However, HGF inhibited Fas-mediated growth suppression and DNA fragmentation in the cells without any increase in Fas antigen expression on the cells. This finding suggests that HGF suppresses apoptotic susceptibility of the cells. From these results, we conclude that the endometrial cytokines may play a role in reshaping the endometrium after menstruation or in regulating apoptotic susceptibility in endometrial epithelium in the mid- to late-secretory period.
巻・号 47(2)
ページ 105-19
公開日 2000-7-1
DOI 10.1016/s0165-0378(00)00062-0
PII S0165-0378(00)00062-0
PMID 10924745
MeSH Apoptosis* Cell Line Cytokines / pharmacology* Endometrium / cytology Endometrium / drug effects* Epidermal Growth Factor / pharmacology Epithelial Cells / cytology Epithelial Cells / drug effects* Female Hepatocyte Growth Factor / pharmacology Humans Interleukin-1 / pharmacology Signal Transduction Transforming Growth Factor beta / pharmacology fas Receptor / metabolism
IF 4.018
引用数 20
WOS 分野 IMMUNOLOGY REPRODUCTIVE BIOLOGY
リソース情報
ヒト・動物細胞 HHUA(RCB0658)