RRC ID 39782
Author Kawanishi M, Enya T, Suzuki H, Takebe H, Matsui S, Yagi T.
Title Postlabelling analysis of DNA adducts formed in human hepatoma cells treated with 3-nitrobenzanthrone.
Journal Mutat. Res.
Abstract 3-Nitrobenzanthrone (NBA) is one of the most mutagenic nitroaromatic compounds that has been found recently in diesel exhaust and airborne particles. A [32P]-postlabelling analysis was carried out to examine the adducts in DNA from human hepatoma HepG2 cells treated with NBA. Two major and two minor adduct spots were obtained in the analysis. The structure of the compound obtained from one of the minor adduct spots was identified to be N-acetyl-3-amino-2-(2'-deoxyguanosin-3', 5'-bisphosphate-8-yl)-benzanthrone, based on identical mobility of the compound with that of synthetic standards in thin-layer chromatography and high performance liquid chromatography. This substance is the identical adduct found in our previous in vitro study. The yet-unidentified major adduct spots may be guanosin- and adenosin-benzanthrone adducts without the N-acetyl group.
Volume 470(2)
Pages 133-9
Published 2000-10-31
DOI 10.1016/s1383-5742(00)00053-3
PII S1383574200000533
PMID 11027967
MeSH Benz(a)Anthracenes / pharmacology* Carcinoma, Hepatocellular / metabolism Carcinoma, Hepatocellular / pathology* Chromatography, High Pressure Liquid Chromatography, Thin Layer DNA Adducts* Humans Liver Neoplasms / metabolism Liver Neoplasms / pathology* Mutagens / pharmacology*
IF 2.011
Times Cited 31
WOS Category TOXICOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY
Resource
Human and Animal Cells