RRC ID 39791
Author Ohnishi T, Suwa M, Oyama T, Arakaki N, Torii M, Daikuhara Y.
Title Prostaglandin E2 predominantly induces production of hepatocyte growth factor/scatter factor in human dental pulp in acute inflammation.
Journal J Dent Res
Abstract Hepatocyte growth factor (HGF), which is also known as the scatter factor, is a broad-spectrum and multifunctional cytokine, mediates epithelial-mesenchyme interaction, and is shown to be involved in the development and regeneration of various tissues, including tooth. Here, we report that HGF was present in adult human dental pulps, and its levels increased during acute inflammation of the tissue. Levels of HGF mRNA in dental pulps also increased with inflammation, as determined by reverse-transcription/polymerase chain-reaction. The production of HGF in fibroblasts from dental pulps in culture was dose-dependently stimulated by inflammatory cytokines such as interleukin (IL)-1alpha and tumor necrosis factor (TNF)-alpha, and by prostaglandin (PG) E2, as determined by an enzyme-linked immunosorbent assay. We also showed that indomethacin did not affect the increase in HGF production by the cells with IL-1alpha, TNF-alpha, and PGE2. The levels of HGF mRNA in the cells were simultaneously increased by these stimulants, as determined by Northern blotting. Since the production of PGs is known to increase at the beginning of inflammation, PGE2 may be involved in the regeneration of dental pulps by the induction of HGF expression after inflammation.
Volume 79(2)
Pages 748-55
Published 2000-2-1
DOI 10.1177/00220345000790020801
PMID 10728976
MeSH Adolescent Adult Anti-Inflammatory Agents, Non-Steroidal / pharmacology Blotting, Northern Cells, Cultured Child Dental Pulp / cytology Dental Pulp / drug effects* Dental Pulp / metabolism Dinoprostone / administration & dosage Dinoprostone / pharmacology* Dose-Response Relationship, Drug Enzyme-Linked Immunosorbent Assay Fibroblasts / drug effects Fibroblasts / metabolism Hepatocyte Growth Factor / biosynthesis* Hepatocyte Growth Factor / genetics Humans Indomethacin / pharmacology Inflammation Mediators / administration & dosage Inflammation Mediators / pharmacology* Interleukin-1 / administration & dosage Interleukin-1 / pharmacology Middle Aged Polymerase Chain Reaction Pulpitis / metabolism* Pulpitis / pathology RNA, Messenger / drug effects RNA, Messenger / genetics Regeneration Tumor Necrosis Factor-alpha / administration & dosage Tumor Necrosis Factor-alpha / pharmacology
IF 4.914
Times Cited 32
Human and Animal Cells WI-38