RRC ID 39799
著者 Yamazaki R, Hatano H, Aiyama R, Matsuzaki T, Hashimoto S, Yokokura T.
タイトル Diarylheptanoids suppress expression of leukocyte adhesion molecules on human vascular endothelial cells.
ジャーナル Eur J Pharmacol
Abstract Diarylheptanoids possess potent anti-inflammatory properties. However, the mechanism of their action is not fully understood. In this study, we found that three diarylheptanoids, 1-(3, 5-dimethoxy-4-hydroxyphenyl)-7-phenylhept-1-en-3-one (YPE-01), yakuchinone B and demethyl-yakuchinone B, reduced the adhesion of both human monocytic cell line U937 and human eosinophilic cell line EoL-1 cells to tumor necrosis factor-alpha (TNF-alpha)-treated human umbilical vein endothelial cells. In addition, they suppressed interleukin-1beta- or TNF-alpha-induced expression of E-selectin, vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) on the surface of the endothelial cells. Since YPE-01 reduced both VCAM-1 and ICAM-1 mRNA induction in TNF-alpha-stimulated endothelial cells, diarylheptanoids appeared to suppress adhesion molecule expression at the transcriptional level. Furthermore, YPE-01 suppressed both VCAM-1 and ICAM-1 mRNA induction as well as edema in 12-O-tetradecanoylphorbol 13-acetate (TPA)-inflamed mice ears in vivo. These results suggest that the anti-inflammatory action of diarylheptanoids is, at least in part, due to their suppressive effect on the surface expression of inducible adhesion molecules in endothelial cells, and subsequent leukocyte adhesion.
巻・号 404(3)
ページ 375-85
公開日 2000-9-22
DOI 10.1016/s0014-2999(00)00620-8
PII S0014-2999(00)00620-8
PMID 10996603
MeSH Animals Cell Adhesion Molecules / drug effects* Cell Adhesion Molecules / metabolism Cells, Cultured Diarylheptanoids* E-Selectin / drug effects E-Selectin / metabolism Edema / drug therapy Edema / metabolism Endothelium, Vascular / drug effects* Endothelium, Vascular / metabolism Guaiacol / analogs & derivatives* Guaiacol / pharmacology Guaiacol / therapeutic use Humans Intercellular Adhesion Molecule-1 / drug effects Intercellular Adhesion Molecule-1 / metabolism Male Mice Mice, Inbred ICR Phytotherapy Plants, Medicinal / therapeutic use Tumor Cells, Cultured Tumor Necrosis Factor-alpha / pharmacology Umbilical Veins / drug effects Umbilical Veins / metabolism Vascular Cell Adhesion Molecule-1 / drug effects Vascular Cell Adhesion Molecule-1 / metabolism
IF 3.263
引用数 16
WOS 分野 PHARMACOLOGY & PHARMACY
リソース情報
ヒト・動物細胞