論文 - 詳細
| RRC ID | 39800 |
|---|---|
| 著者 | Nakayama Y, Aoki Y. |
| タイトル | Mechanism responsible for the formation of focal swellings on injured neuronal processes using a novel in vitro model of axonal injury. |
| ジャーナル | Forensic Sci Int |
| Abstract |
A novel in vitro model of axonal injury using PC12 cells was designed to introduce traumatic alterations on neuronal processes and to identify mechanisms responsible for the formation of focal swellings by observation with phase-contrast and transmission electron microscopes. The injury on the processes was developed by one-dimensional, horizontal oscillation. Phase-contrast microscopic observation on the injured processes showed their terminal increase in diameter. Long term observation of cellular responses to the mechanical insult disclosed that the terminal swelling coincided with the detachment of growth cones from the culture plate. The finding suggests that the detachment of the growth cone would destroy a cytoskeletal network, which determines and maintains the cell shapes, and then cause the spherical deformation of the processes. Ultramicroscopically, the processes with terminal swellings regenerated the growth cones by the cytoskeletal reconstruction. |
| 巻・号 | 113(1-3) |
| ページ | 245-9 |
| 公開日 | 2000-9-11 |
| DOI | 10.1016/s0379-0738(00)00217-6 |
| PII | S0379073800002176 |
| PMID | 10978633 |
| MeSH | Animals Biomechanical Phenomena Cell Culture Techniques / methods Cytoskeleton / pathology Cytoskeleton / ultrastructure Diffuse Axonal Injury / etiology* Diffuse Axonal Injury / pathology* Disease Models, Animal* Growth Cones / pathology Growth Cones / ultrastructure Microscopy, Electron, Scanning Transmission Microscopy, Phase-Contrast PC12 Cells* Rats |
| IF | 2.108 |
| 引用数 | 4 |
| WOS 分野 | MEDICINE, LEGAL |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | PC-12(RCB0009) |