| RRC ID |
39810
|
| Author |
Takahashi S, Takeda E, Kubota Y, Okayasu R.
|
| Title |
Inhibition of repair of radiation-induced DNA double-strand breaks by nickel and arsenite.
|
| Journal |
Radiat Res
|
| Abstract |
The effect of arsenite or nickel on the repair of DNA double-strand breaks (DSBs) was studied in gamma-irradiated Chinese hamster ovary cells using pulsed-field gel electrophoresis. After treatment with nickel chloride or arsenite for 2 h, cells were irradiated with gamma rays at a dose of 40 Gy, and the numbers of DNA DSBs were measured immediately after irradiation as well as at 30 min postirradiation. Both arsenite and nickel(II) inhibited repair of DNA DSBs in a concentration-dependent manner; 0.08 mM arsenite significantly inhibited the rejoining of DSBs, while 76 mM nickel was necessary to observe a clear inhibition. The mean lethal concentrations for the arsenite and nickel(II) treatments were approximately 0.12 and 13 mM, respectively. This indicates that the inhibition of repair by arsenite occurred at a concentration at which appreciable cell survival occurred, but that nickel(II) inhibited repair only at cytotoxic concentrations at which the cells lost their proliferative ability. These novel observations provide insight into the mechanisms underlying the effects of combined exposure to arsenite and ionizing radiation in our environment.
|
| Volume |
154(6)
|
| Pages |
686-91
|
| Published |
2000-12-1
|
| DOI |
10.1667/0033-7587(2000)154[0686:iorori]2.0.co;2
|
| PMID |
11096426
|
| MeSH |
Animals
Arsenites / pharmacology*
CHO Cells / drug effects*
CHO Cells / metabolism
CHO Cells / radiation effects
Cell Adhesion / drug effects
Cell Survival / drug effects
Cricetinae
DNA / analysis
DNA / metabolism
DNA / radiation effects
DNA Damage*
DNA Repair / drug effects*
Dose-Response Relationship, Drug
Gamma Rays
Nickel / pharmacology*
|
| IF |
2.657
|
| Times Cited |
19
|
|
WOS Category
|
RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
BIOLOGY
BIOPHYSICS
|
| Resource |
| Human and Animal Cells |
CHO-K1(RCB0285) |
