| RRC ID |
39818
|
| 著者 |
Tanimura A, Tojyo Y, Turner RJ.
|
| タイトル |
Evidence that type I, II, and III inositol 1,4,5-trisphosphate receptors can occur as integral plasma membrane proteins.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
A number of previous reports have suggested that inositol 1,4, 5-trisphosphate receptors (IP(3)Rs) are present in the plasma membranes of cells. We confirm this directly in the present study by demonstrating that a significant proportion of the IP(3)Rs found in A431 cells, Jurkat cells, and rat parotid acini can be biotinylated by the extracellular application of sulfo-N-hydroxysuccinimide-biotin to intact cells. This labeling cannot be accounted for by the reaction of sulfo-N-hydroxysuccinimide-biotin with intracellular IP(3)Rs since calnexin and the SERCA2 ATPase, both integral membrane proteins of the endoplasmic reticulum, are not labeled under the same experimental conditions. Individual IP(3)R subtypes were detected using subtype-specific antibodies. A431 cells expressed only the type-3 IP(3)R, and 23% of this protein was in the biotinylated (plasma membrane) fraction. Jurkat cells and rat parotid cells expressed all three IP(3)R subtypes. Contrary to earlier results suggesting that only the type-3 IP(3)R might localize to the plasma membrane, we found that significant amounts (5-14%) of all three subtypes could be identified in the biotinylated fractions of Jurkat and rat parotid cells. Our results suggest a role for IP(3)Rs in plasma membrane as well as intracellular membrane function.
|
| 巻・号 |
275(35)
|
| ページ |
27488-93
|
| 公開日 |
2000-9-1
|
| DOI |
10.1074/jbc.M004495200
|
| PII |
S0021-9258(19)61534-6
|
| PMID |
10874040
|
| MeSH |
Amino Acid Sequence
Animals
Antibodies / immunology
Calcium Channels / immunology
Calcium Channels / metabolism*
Humans
Inositol 1,4,5-Trisphosphate Receptors
Jurkat Cells
Membrane Proteins / metabolism*
Molecular Sequence Data
Parotid Gland / cytology
Parotid Gland / metabolism
Protein Isoforms / immunology
Protein Isoforms / metabolism*
Rats
Receptors, Cytoplasmic and Nuclear / immunology
Receptors, Cytoplasmic and Nuclear / metabolism*
Tumor Cells, Cultured
|
| IF |
4.238
|
| 引用数 |
51
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
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| リソース情報 |
| ヒト・動物細胞 |
|
