論文 - 詳細
| RRC ID | 39823 |
|---|---|
| 著者 | Kurosawa N, Kadomatsu K, Ikematsu S, Sakuma S, Kimura T, Muramatsu T. |
| タイトル | Midkine binds specifically to sulfatide the role of sulfatide in cell attachment to midkine-coated surfaces. |
| ジャーナル | Eur J Biochem |
| Abstract |
Midkine is a heparin-binding polypeptide which is implicated in the control of development and repair of various tissues. Recognition of sulfate groups in glycosaminoglycans is important for its function. To elucidate further its mechanism of action, the interactions of midkine with sulfated glycolipids were studied. Of various glycolipids and lipids examined, midkine bound strongly to sulfatide and cholesterol-3-sulfate (CHO-3-SO4) in a dose-dependent manner but failed to bind to other standard glycolipids and lipids. The properties of midkine binding to sulfatide and to CHO-3-SO4 differed in their sensitivity to inhibition by anionic polysaccharides, salt concentration and unlabeled midkine. Heparin inhibited midkine binding to sulfatide but weakly inhibited its binding to CHO-3-SO4. Liposomes bearing sulfatide carried out significant interactions with immobilized midkine, whereas those bearing CHO-3-SO4 did not. Incorporation of sulfatide into 32D cells and trypsinized COS cells enhanced 125I-labelled midkine binding, whereas incorporation of ganglioside or galactosylceramide had no effect. Furthermore, sulfatide-incorporated cells enhanced cell attachment to midkine-coated coverslips. These results indicate that midkine binds to sulfatide under physiological conditions and the midkine-sulfatide interaction may be important in controlling cell attachment. |
| 巻・号 | 267(2) |
| ページ | 344-51 |
| 公開日 | 2000-1-1 |
| DOI | 10.1046/j.1432-1327.2000.01005.x |
| PII | ejb1005 |
| PMID | 10632704 |
| MeSH | Adsorption Animals COS Cells / cytology COS Cells / metabolism Carrier Proteins / metabolism* Cell Adhesion / physiology Cell Membrane / metabolism Cytokines* Liposomes / metabolism Midkine Sulfoglycosphingolipids / metabolism* Surface Properties |
| 引用数 | 17 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 32D(RCB1145) COS-7(RCB0539) |