RRC ID |
39847
|
Author |
Hashimoto N, Araki N, Yoshikawa H, Myoui A, Matsumine A, Kaneko M, Sonobe H, Ochi T.
|
Title |
SYT-SSX fusion proteins in synovial sarcomas: detection and characterization with new antibodies.
|
Journal |
Cancer Lett
|
Abstract |
To identify and characterize the SYT-SSX fusion proteins in synovial sarcomas, we developed two polyclonal antibodies against the N-terminal part and for the C-terminal part of the SYT-SSX2 protein. Specificity was demonstrated on COS-7 cells transfected with two subtypes of SYT-SSX fusion genes, SYT-SSX1 and SYT-SSX2. Both antibodies recognized a single protein of 61 kDa in an immunoprecipitation of the transfected COS-7 cell lysates. These antibodies also detected the native protein of 61 kDa in the lysate of a human synovial sarcoma cell line (HS-SY41) with immunoprecipitation, and in extracts of human synovial sarcomas with western blot analysis. An immunohistochemical study, using human synovial sarcoma tissues, demonstrated that the SYT-SSX fusion proteins localized in the nucleus of the tumor cells. These antibodies provide a useful method for studying the expression of the SYT-SSX fusion proteins.
|
Volume |
149(1-2)
|
Pages |
31-6
|
Published |
2000-2-28
|
DOI |
10.1016/s0304-3835(99)00336-5
|
PII |
S0304-3835(99)00336-5
|
PMID |
10737705
|
MeSH |
Antibodies, Neoplasm / immunology*
Antibody Specificity
Biomarkers, Tumor* / analysis
Humans
Immunohistochemistry
Oncogene Proteins, Fusion / analysis
Oncogene Proteins, Fusion / immunology
Oncogene Proteins, Fusion / metabolism*
Sarcoma, Synovial / immunology
Sarcoma, Synovial / metabolism*
|
IF |
7.36
|
Times Cited |
10
|
WOS Category
|
ONCOLOGY
|
Resource |
Human and Animal Cells |
COS-7(RCB0539) |