RRC ID 39873
Author Okui M, Ito F, Ogita K, Kuramoto N, Kudoh J, Shimizu N, Ide T.
Title Expression of APG-2 protein, a member of the heat shock protein 110 family, in developing rat brain.
Journal Neurochem Int
Abstract APG-2 protein is a member of the heat shock protein 110 family, and it is thought to play an important role in the maintenance of neuronal functions under physiological and stress conditions. However, neither the tissue-distribution of APG-2 protein nor developmental change of its expression has been studied at the protein level. Therefore, we generated an antiserum against APG-2 protein and studied expression of this protein in rat brain and other tissues by use of the Western blot method. The results showed a high expression of APG-2 protein in various regions of the central nervous system (cerebral cortex, hippocampus, striatum, midbrain, hypothalamus, cerebellum, medulla pons, and spinal cord) throughout the entire postnatal stage. Similarly, a high level of APG-2 protein was detected in the whole brain of rat embryos and in adult rat tissues such as liver, lung, spleen, and kidney. In contrast, its expression in heart was high at postnatal days 1 and 3, but thereafter drastically decreased to a low level. Furthermore, APG-2 protein was detected in neuronal primary cultures prepared from rat cerebral cortex, and its level did not change notably during neuronal differentiation. These results show that APG-2 protein is constitutively expressed in various tissues and also in neuronal cells throughout the entire embryonic and postnatal period. suggesting that it might play an important role in these tissues under non-stress conditions.
Volume 36(1)
Pages 35-43
Published 2000-1-1
DOI 10.1016/s0197-0186(99)00095-9
PII S0197-0186(99)00095-9
PMID 10566957
MeSH Aging / metabolism* Amino Acid Sequence Animals Animals, Newborn Brain / embryology Brain / growth & development Brain / metabolism* Embryonic and Fetal Development / physiology* Female Gene Expression Regulation, Developmental* HSP110 Heat-Shock Proteins HSP70 Heat-Shock Proteins / chemistry Heat-Shock Proteins / chemistry Heat-Shock Proteins / genetics* Heat-Shock Proteins / isolation & purification Immune Sera Male Molecular Sequence Data Organ Specificity Peptide Fragments / immunology Rats Rats, Wistar Sequence Alignment Sequence Homology, Amino Acid
IF 3.881
Times Cited 6
Human and Animal Cells PC-12(RCB0009)