| RRC ID |
39874
|
| 著者 |
Hara H, Uchida S, Yoshimura H, Aoki M, Toyoda Y, Sakai Y, Morimoto S, Fukamachi H, Shiokawa K, Hanada K.
|
| タイトル |
Isolation and characterization of a novel liver-specific gene, hepassocin, upregulated during liver regeneration.
|
| ジャーナル |
Biochim Biophys Acta
|
| Abstract |
By differential cDNA cloning coupled with Xenopus oocyte expression screening, we isolated a cDNA encoding a novel protein, termed 'hepassocin', the expression of which is upregulated in the regenerating rat liver. The cDNA contained a single open reading frame encoding a protein of 314 amino acids (ca. 34 kDa), including 24 amino acids of signal sequence. The protein expressed from the cDNA in Verots cells had activity to stimulate DNA synthesis in primary rat hepatocytes and was of 66 kDa or 34 kDa, under non-reducing or reducing conditions, respectively. Using an affinity column conjugated with the antibody raised against a peptide in a hydrophilic region, we purified hepassocin from the rat liver: it had a DNA synthesis-stimulating activity in hepatocytes. The hepassocin obtained here was 66 kDa, and the 34 kDa protein obtained under reducing conditions contained five cysteine residues, indicating that hepassocin is active as a homodimer. Northern blot analysis revealed that hepassocin mRNA (1.4 kb in length) occurred only in the liver, and in situ hybridization studies revealed its presence in parenchymal hepatocytes but not in endothelial cells. Furthermore, the expression of hepassocin mRNA was upregulated during compensatory hyperplasia after partial hepatectomy and regeneration after galactosamine treatment in the rat liver. These results suggest that hepassocin plays an important role in stimulating liver cell growth, through an autocrine mechanism.
|
| 巻・号 |
1492(1)
|
| ページ |
31-44
|
| 公開日 |
2000-6-21
|
| DOI |
10.1016/s0167-4781(00)00056-7
|
| PII |
S0167-4781(00)00056-7
|
| PMID |
11004478
|
| MeSH |
Amino Acid Sequence
Animals
Base Sequence
DNA / biosynthesis
DNA, Complementary / analysis
DNA, Complementary / genetics
Galactosamine
Gene Expression Regulation*
Liver / metabolism*
Liver Failure, Acute / chemically induced
Liver Failure, Acute / metabolism
Liver Regeneration / genetics*
Liver Regeneration / physiology
Male
Molecular Sequence Data
Neoplasm Proteins*
Proteins / genetics*
Proteins / isolation & purification
RNA, Messenger / metabolism
Rats
Rats, Sprague-Dawley
Recombinant Proteins / analysis
Transfection
Up-Regulation
|
| IF |
3.411
|
| 引用数 |
24
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
Verots S3(RCB0707) |
