論文 - 詳細
| RRC ID | 39893 |
|---|---|
| 著者 | Fukushima K, Yamashita K. |
| タイトル | Interleukin-2 carbohydrate recognition modulates CTLL-2 cell proliferation. |
| ジャーナル | J Biol Chem |
| Abstract |
Interleukin-2 (IL-2) specifically recognizes high-mannose type glycans with five or six mannosyl residues. To determine whether the carbohydrate recognition activity of IL-2 contributes to its physiological activity, the inhibitory effects of high-mannose type glycans on IL-2-dependent CTLL-2 cell proliferation were investigated. Man(5)GlcNAc(2)Asn added to CTLL-2 cell cultures inhibited not only phosphorylation of tyrosine kinases but also IL-2-dependent cell proliferation. We found that a complex of IL-2, IL-2 receptor alpha, beta, gamma subunits, and tyrosine kinases was formed in rhIL-2-stimulated CTLL-2 cells. Among the components of this complex, only the IL-2 receptor alpha subunit was stained with Galanthus nivalis agglutinin which specifically recognizes high-mannose type glycans. This staining was diminished after digestion of the glycans with endo-beta-N-acetylglucosaminidase H or D, suggesting that at least a N-glycan containing Man(5)GlcNAc(2) is linked to the extracellular portion of the IL-2 receptor alpha subunit. Our findings indicate that IL-2 binds the IL-2 receptor alpha subunit through Man(5)GlcNAc(2) and a specific peptide sequence on the surface of CTLL-2 cells. When IL-2 binds to the IL-2Ralpha subunit, this may trigger formation of the high affinity complex of IL-2-IL-2Ralpha, -beta, and -gamma subunits, leading to cellular signaling. |
| 巻・号 | 276(10) |
| ページ | 7351-6 |
| 公開日 | 2001-3-9 |
| DOI | 10.1074/jbc.M008781200 |
| PII | S0021-9258(19)67186-3 |
| PMID | 11076950 |
| MeSH | Animals Carbohydrate Metabolism* Cell Division Dose-Response Relationship, Drug Galanthus Humans Interleukin-2 / metabolism* Interleukin-2 / physiology* Janus Kinase 1 Janus Kinase 3 Lymphocyte Specific Protein Tyrosine Kinase p56(lck) / metabolism Mannose / metabolism Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase / metabolism Mice Models, Biological Phosphorylation Polysaccharides / metabolism Precipitin Tests Protein-Tyrosine Kinases / metabolism Receptors, Interleukin-2 / chemistry Receptors, Interleukin-2 / metabolism Recombinant Proteins / metabolism Signal Transduction T-Lymphocytes / metabolism src-Family Kinases / metabolism |
| IF | 4.238 |
| 引用数 | 18 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | CTLL-2(RCB0637) |