Reference - Detail
RRC ID | 39897 |
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Author | Fujita T, Meguro T, Izumo N, Yasutomi C, Fukuyama R, Nakamuta H, Koida M. |
Title | Phosphate stimulates differentiation and mineralization of the chondroprogenitor clone ATDC5. |
Journal | Jpn J Pharmacol |
Abstract |
ATDC5 cells were employed to examine how inorganic phosphate (Pi) influences chondrocytic bone formation. 1) Pi (3 - 30 mM) plus ascorbic acid (50 microg/ml) dose-dependently accelerated proliferative differentiation and mineralization of ATDC5. 2) Northern blot analysis revealed that 10 mM Pi suppressed expression of type II collagen and PTH (parathyroid hormone) / PTH-related peptide (PTHrP) receptor, while it accelerated type X collagen expression. 3) Pi (3 - 30 mM) dose-dependently increased luciferase activity in the cells transfected with 3000 bp type X collagen promoter fused to the luciferase gene. The results suggest a regulatory role of Pi in endochondral osteogenesis. |
Volume | 85(3) |
Pages | 278-81 |
Published | 2001-3-1 |
DOI | 10.1254/jjp.85.278 |
PMID | 11325020 |
MeSH | Animals Biomarkers / analysis Blotting, Northern Calcification, Physiologic / physiology* Cell Differentiation Chondrocytes / cytology* Chondrocytes / metabolism Clone Cells Collagen / metabolism Mice Parathyroid Hormone / metabolism Parathyroid Hormone-Related Protein Phosphates / metabolism* Phosphates / pharmacology Proteins / metabolism Stem Cells / cytology* Stem Cells / metabolism |
Times Cited | 22 |
WOS Category | PHARMACOLOGY & PHARMACY |
Resource | |
Human and Animal Cells | ATDC5(RCB0565) |