論文 - 詳細
| RRC ID | 39897 |
|---|---|
| 著者 | Fujita T, Meguro T, Izumo N, Yasutomi C, Fukuyama R, Nakamuta H, Koida M. |
| タイトル | Phosphate stimulates differentiation and mineralization of the chondroprogenitor clone ATDC5. |
| ジャーナル | Jpn J Pharmacol |
| Abstract |
ATDC5 cells were employed to examine how inorganic phosphate (Pi) influences chondrocytic bone formation. 1) Pi (3 - 30 mM) plus ascorbic acid (50 microg/ml) dose-dependently accelerated proliferative differentiation and mineralization of ATDC5. 2) Northern blot analysis revealed that 10 mM Pi suppressed expression of type II collagen and PTH (parathyroid hormone) / PTH-related peptide (PTHrP) receptor, while it accelerated type X collagen expression. 3) Pi (3 - 30 mM) dose-dependently increased luciferase activity in the cells transfected with 3000 bp type X collagen promoter fused to the luciferase gene. The results suggest a regulatory role of Pi in endochondral osteogenesis. |
| 巻・号 | 85(3) |
| ページ | 278-81 |
| 公開日 | 2001-3-1 |
| DOI | 10.1254/jjp.85.278 |
| PMID | 11325020 |
| MeSH | Animals Biomarkers / analysis Blotting, Northern Calcification, Physiologic / physiology* Cell Differentiation Chondrocytes / cytology* Chondrocytes / metabolism Clone Cells Collagen / metabolism Mice Parathyroid Hormone / metabolism Parathyroid Hormone-Related Protein Phosphates / metabolism* Phosphates / pharmacology Proteins / metabolism Stem Cells / cytology* Stem Cells / metabolism |
| 引用数 | 22 |
| WOS 分野 | PHARMACOLOGY & PHARMACY |
| リソース情報 | |
| ヒト・動物細胞 | ATDC5(RCB0565) |