Reference - Detail
|Author||Shoji Y, Inoue Y, Sugisawa H, Itou T, Endo T, Sakai T.|
|Title||Molecular cloning and functional characterization of bottlenose dolphin (Tursiops truncatus) tumor necrosis factor alpha.|
|Journal||Vet Immunol Immunopathol|
Bottlenose dolphin tumor necrosis factor alpha (doTNF-alpha) cDNA was cloned by reverse transcription polymerase chain reaction (RT-PCR) and the nucleic and deduced amino acid sequences were determined. The sequence of the cDNA clones shows that doTNF-alpha has an open reading frame of 699bp encoding 233 amino acids. The nucleic acid sequence of doTNF-alpha indicates 90, 88, 87, and 79% similarity with the cattle, pig, human, and mouse TNF-alpha gene, respectively. Based on the analysis of human and mouse TNF-alpha molecules, doTNF-alpha is processed to a mature protein with 157 amino acids. The 233 amino acids precursor has a hydrophobic region that could serve as a transmembrane domain. The recombinant doTNF-alpha expressed in Escherichia coli as a glutathione S-transferase fusion protein reacted with anti-human TNF-alpha antibody and exerted cytotoxity to the TNF-alpha sensitive murine cell line L929.
|MeSH||Amino Acid Sequence Animals Base Sequence Biological Assay / veterinary Cells, Cultured DNA / chemistry Dolphins / genetics* Dolphins / immunology* Escherichia coli / genetics Mice Molecular Sequence Data RNA / chemistry RNA / isolation & purification Random Amplified Polymorphic DNA Technique / veterinary Recombinant Proteins / biosynthesis Recombinant Proteins / genetics Recombinant Proteins / toxicity Reverse Transcriptase Polymerase Chain Reaction / veterinary Sequence Homology, Amino Acid Tumor Necrosis Factor-alpha / genetics* Tumor Necrosis Factor-alpha / physiology Tumor Necrosis Factor-alpha / toxicity|
|WOS Category||VETERINARY SCIENCES IMMUNOLOGY|
|Human and Animal Cells|