RRC ID 39900
Author Shoji Y, Inoue Y, Sugisawa H, Itou T, Endo T, Sakai T.
Title Molecular cloning and functional characterization of bottlenose dolphin (Tursiops truncatus) tumor necrosis factor alpha.
Journal Vet Immunol Immunopathol
Abstract Bottlenose dolphin tumor necrosis factor alpha (doTNF-alpha) cDNA was cloned by reverse transcription polymerase chain reaction (RT-PCR) and the nucleic and deduced amino acid sequences were determined. The sequence of the cDNA clones shows that doTNF-alpha has an open reading frame of 699bp encoding 233 amino acids. The nucleic acid sequence of doTNF-alpha indicates 90, 88, 87, and 79% similarity with the cattle, pig, human, and mouse TNF-alpha gene, respectively. Based on the analysis of human and mouse TNF-alpha molecules, doTNF-alpha is processed to a mature protein with 157 amino acids. The 233 amino acids precursor has a hydrophobic region that could serve as a transmembrane domain. The recombinant doTNF-alpha expressed in Escherichia coli as a glutathione S-transferase fusion protein reacted with anti-human TNF-alpha antibody and exerted cytotoxity to the TNF-alpha sensitive murine cell line L929.
Volume 82(3-4)
Pages 183-92
Published 2001-10-1
DOI 10.1016/s0165-2427(01)00353-1
PII S0165242701003531
PMID 11587733
MeSH Amino Acid Sequence Animals Base Sequence Biological Assay / veterinary Cells, Cultured DNA / chemistry Dolphins / genetics* Dolphins / immunology* Escherichia coli / genetics Mice Molecular Sequence Data RNA / chemistry RNA / isolation & purification Random Amplified Polymorphic DNA Technique / veterinary Recombinant Proteins / biosynthesis Recombinant Proteins / genetics Recombinant Proteins / toxicity Reverse Transcriptase Polymerase Chain Reaction / veterinary Sequence Homology, Amino Acid Tumor Necrosis Factor-alpha / genetics* Tumor Necrosis Factor-alpha / physiology Tumor Necrosis Factor-alpha / toxicity
IF 1.713
Times Cited 18
Human and Animal Cells